FastQCFastQC Report
Wed 25 May 2016
SRR1780940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414328
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213345.149060647602866No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG21640.5222915178312834No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTT200.0069598752.4790889
TGGTCGC3350.048.0521649
CGGTGGT3600.047.6199246
TCGCCGT3350.047.00754552
GCCGTAT3350.047.00754554
TCGGTGG3500.046.99262245
GTCGCCG3450.046.6593451
TAGACTC305.8865733E-446.648084
CTCGGTG3550.045.34499744
ATCTCGG3400.045.29794342
GATCTCG3600.042.78656441
AGATCTC3700.042.57630540
GTAGATC3800.042.3771238
TAGATCT3850.041.82676739
TCTCGGT3950.040.75309843
CGCCGTA3900.039.48098453
CGTATCA4300.038.24519356
GTGGTCG4400.038.16661548
GGTGGTC4700.036.47482747
GTATCAT4350.036.19684257
Produced by FastQC (version 0.11.5)