FastQCFastQC Report
Wed 25 May 2016
SRR1780940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414328
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT91192.2009132860921783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39360.9499720028576395No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT29390.7093413913614334No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG19010.4588152381687938No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT7420.1790851692378985RNA PCR Primer, Index 47 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT150.00222154170.01871537
AATGTCG150.00222154170.01871568
CGTCTTA453.8562575E-1062.21632848
AGGGGGG19400.053.95771870
CACGTAT200.006941572352.51403439
CGTCACT200.006941572352.51403437
CAGATCG200.006951521652.495027
CCGTCTT20850.050.85848647
GCCGTCT21450.050.57795346
ATCTCGT21650.050.45228237
TATGCCG22100.050.3572243
GATCTCG21700.050.33603736
CGTATGC22450.050.19568341
CGTCTTC20950.050.1145848
GTAGATC22000.049.94980633
CTCGTAT22100.049.90021539
AGATCTC21850.049.83025435
TAGATCT21900.049.71648434
GTATGCC22400.049.6827942
TCGTATG22900.049.53288740