FastQCFastQC Report
Wed 25 May 2016
SRR1780939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150119
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3646524.290729354711928No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG54173.608470613313438No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT6210.41367182035585104No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4790.3190801963775405No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG4290.2857732865260227No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2160.14388585055855688No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG150.00222297369.9853444
TATACTG150.00222297369.9853445
GCGCTGC650.059.23810215
ACTCTGG252.3828626E-456.0069361
CGAACGA200.00693692852.50649614
CAAGTGG200.00693692852.50649647
GCTGCGA200.00693692852.50649669
TTGAGCT200.00693692852.50649669
ACGATTT200.00693692852.50649630
TTGACGC200.00693692852.50649645
TTGAACC200.00693692852.50649634
CGACTAA200.00693692852.50649661
ATGCCAT200.00693692852.50649645
GCAGACT200.00693692852.50649662
TCAACGT200.00693692852.50649668
AGCGTTT200.006946077652.48900611
AACCGAC200.006946077652.48900658
AACTCTG200.006946077652.48900660
GGTAGTG200.006946077652.48900658
TCGCCGT7500.051.7891552