FastQCFastQC Report
Wed 25 May 2016
SRR1780939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150119
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1981413.19886223595947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87375.82004942745422No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT35502.3647905994577636No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG34012.2655360081002405No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT10990.7320858785363611Illumina PCR Primer Index 9 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC10860.7234260819749665No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC5870.3910231216568189No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3300.21982560502001747No Hit
CTATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2890.19251393894177285No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1900.12656625743576763No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT1870.12456784284467655No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT1790.1192387372684337No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT1700.11324349349516051No Hit
TATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1680.11191121710109979No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1660.1105789407070391No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1530.10191914414564446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAG150.002221127370.0000114
TCGTATA150.002221127370.0000140
GTATACC150.002221127370.0000142
GCGGATG150.002221127370.0000148
CCGAAGC150.002221127370.0000123
CGCCGAG150.002221127370.0000131
AGGGGGG32650.063.8897470
CGTCTAC453.8198777E-1062.22222548
ACGTATG401.0588337E-861.2540
CGTATGA1200.058.33333641
CACGTAT308.344796E-658.33333639
ATCGAAT308.344796E-658.33333639
AGACGAT1150.057.8260925
CGAATGC553.45608E-1157.27272441
AGCCGTC509.731593E-1055.99999645
ATATCGA252.384323E-455.99999637
TCACTCG252.384323E-455.99999653
ACTCGAG252.384323E-455.99999655
CTCGAGA252.384323E-455.99999656
TGACGTC1150.054.7826145
Produced by FastQC (version 0.11.5)