FastQCFastQC Report
Wed 25 May 2016
SRR1780938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237476
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2986512.576007680776163No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG40481.7045933062709497No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT4590.1932826896191615No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4260.17938654853543096No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2790.11748555643517661No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGA150.00222142570.0103267
GGTCATA150.00222328269.9955751
GGGCGCT150.0022251469.98083513
TGGGCGC207.926428E-569.9808353
GGCGCGT150.0022251469.9808355
CTCGGTG5850.053.24449544
ATCTCGG5750.052.96433342
GATCTCG5750.052.96433341
AATCTTG200.00693544552.51880620
AGTGGTC200.006941227252.50774441
GGCAGTC200.00694701352.49668551
CCCAGGT200.00694701352.49668563
CCAACGG200.00694701352.49668563
TCCCCAG200.00694701352.49668561
GCCGTAT6000.052.49668554
TTGGGCG200.00694701352.4966852
TGGTCGC5950.052.34963249
CGGTGGT6050.052.06282846
AGATCTC5850.052.05895640
TCGCCGT6000.051.91338752
Produced by FastQC (version 0.11.5)