FastQCFastQC Report
Wed 25 May 2016
SRR1780938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237476
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT149836.309269147198032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63952.6929037039532413No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT34701.4612002897134868No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG26471.1146389529889338No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT9070.3819333322104128RNA PCR Primer, Index 42 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7110.2993986760767404No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4300.18107092927285284No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2780.11706446125082115RNA PCR Primer, Index 32 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCT150.002222725470.041
TAACTCG401.0622898E-861.2530
AGGGGGG27800.060.0539670
ACGTATG308.359719E-658.33333240
AACTCGT308.359719E-658.33333237
AGCCGTC252.3871622E-455.99999645
CACGTAT252.3871622E-455.99999639
AACCGTG200.006945280452.58
TAGAGCC200.006945280452.54
TCTGACC200.006945280452.524
TGAACGT200.006945280452.520
TAACCGT200.006945280452.57
GGTGGAC200.006945280452.530
GAACGTA200.006945280452.521
TGGGGCG200.006945280452.556
CGTACAC200.006945280452.522
GCAGGAC200.006945280452.550
GTGCGTC200.006945280452.554
CTGGCCA200.006945280452.519
ATACCAC200.006945280452.541
Produced by FastQC (version 0.11.5)