FastQCFastQC Report
Wed 25 May 2016
SRR1780937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308791
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4668215.117668584900468No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG66652.1584178295351872No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT7120.23057666836144836No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC6750.2185944538539012No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5380.17422787581244273No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG150.002225584169.9823553
CGTGCGA252.3912676E-455.98587810
GGTCGAC200.006949723752.49526247
CGTATTT200.006954177352.4867636
GTCGACG200.006954177352.48676348
CCGTGCG200.006954177352.4867639
GGTCATA200.006954177352.4867631
CCCGTGC200.006954177352.4867638
GCACTAG200.006954177352.4867631
GACTATA200.006954177352.4867631
TACGTGA200.006954177352.4867635
GATCTCG9900.047.3924941
CTCGGTG10050.047.36658544
GCCGTAT9900.047.3617954
AGATCTC9950.047.14669440
CGGTGGT10200.047.0055646
TACGTAC305.861237E-446.68514323
ATCTCGG10050.046.67757842
GTACTCG305.865921E-446.67757438
TCGCCGT10050.046.654952
Produced by FastQC (version 0.11.5)