FastQCFastQC Report
Wed 25 May 2016
SRR1780937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308791
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT242027.837663662477209No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106173.438247876395361No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT50121.6231043003196337No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG43261.4009475664770021No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT13600.4404273440611935RNA PCR Primer, Index 15 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC10640.3445696280008161No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC7340.23770122833890883No Hit
CTATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4370.14151966864319232No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3870.12532748687623668No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT3390.10978299237995927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA301.0217991E-770.039
GATCTCG207.91945E-570.036
CCCGGTG150.002223359470.023
ATACCAG150.002223359470.06
AGGGGGG39250.061.70700570
ACTGCAT308.365645E-658.33333253
CGTCTAC509.786163E-1055.99999648
TAACCGA252.3882899E-455.99999611
TCGAAAG452.6950147E-854.44444340
CTTTATA1300.053.8461572
GTGTTGC200.006947246452.527
GATGGTC200.006947246452.543
CGGTGTT200.006947246452.525
GGTCTAA200.006947246452.51
TTCGCTA200.006947246452.559
CGATATT200.006947246452.511
GCCGTAT200.006947246452.546
AAGCTTG200.006947246452.554
GCCGTCT47050.051.77470446
CGTCTTC47700.051.43605848
Produced by FastQC (version 0.11.5)