FastQCFastQC Report
Wed 25 May 2016
SRR1780936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503224
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG428498.514895950908542No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG51191.0172408311209322No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT5930.11784016660572628No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC5830.11585297998505635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCGG150.00222257270.01272618
TTAGTGC200.006958445552.4834548
CTCGGTG9300.047.7996444
TCGGTGG9450.047.78171545
CGGTGGT9450.047.41131246
GCCGTAT9250.047.2823954
GTTACCA305.879607E-446.66123663
TCGCCGT9300.046.65196252
GATCTAC305.8853714E-446.651961
GTCGCCG9500.046.40642551
TGGTCGC9500.046.40642549
ATCTCGG9450.045.18890842
GATCTCG9550.044.7201741
AGATCTC9650.044.61507840
GTAGATC9500.044.59148838
TAGATCT9500.044.59148839
TCTCGGT9800.044.2893643
CGCCGTA10050.043.51861653
CGTATCA10050.043.17046756
AGCGTTC659.962605E-943.09760737
Produced by FastQC (version 0.11.5)