FastQCFastQC Report
Wed 25 May 2016
SRR1780936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503224
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT226154.494022542645025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85901.7069933071554615No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG40140.797656709536906No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT39230.7795733112888097No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT13110.2605201659698266Illumina PCR Primer Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC12370.24581498497686913No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC7020.1395005007710284No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAC150.002224776769.995233
AGGGGGG37700.058.30420770
AACTCGT407.4691343E-752.50685537
GGACTTG200.0069516552.4964218
CGTCTTA552.2791937E-950.91573748
CTCGTAT43300.050.44538539
TATGCCG43400.050.32415443
CCGTCTT42100.050.22033347
TCGTATG44150.050.18775640
CGTCTTC42500.050.15949248
TCTCGTA43450.050.1101138
GCCGTCT42550.050.10054846
ATCTCGT42900.050.01818537
TAATTCG352.085402E-550.00156434
CGTATGC43750.050.0015641
TCTTCTG42600.049.9546150
GATCTCG43300.049.79865336
AGATCTC43450.049.70235435
GTATGCC44000.049.5583742
ATGCCGT44000.049.1606344