FastQCFastQC Report
Wed 25 May 2016
SRR1780935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214534
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4245519.789404010553106No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG54932.5604333112700086No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC6080.28340496145133176No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT5090.2372584298992234No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG4190.195307037579125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA150.002221051170.0109613
TACCGTT150.002221051170.0109612
GAGCTCG150.00222310669.99464441
ACATGTT150.00222310669.99464432
TGTGCGC252.8344075E-669.994643
TGCGCGT252.8344075E-669.994645
GCGTGTG207.9169484E-569.994648
ACGGTTA252.3847942E-456.00876650
TGTCGGT252.387544E-455.9957143
TACTCGC200.00694006952.50821720
TCGTTGT200.006946472552.4959834
TTTAACG200.006946472552.4959837
CGACCGG200.006946472552.4959830
TAACGGC200.006946472552.4959839
GTGTCGG200.006946472552.4959842
TTAACGG200.006946472552.4959838
GATCTAC200.006946472552.495981
CGCGTCT407.45953E-752.495987
CGCGTCG200.006946472552.495987
CGGTACC200.006946472552.4959848
Produced by FastQC (version 0.11.5)