##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780935_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 214534 Sequences flagged as poor quality 0 Sequence length 76 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.361756178507836 32.0 27.0 32.0 27.0 32.0 2 29.90587972069695 32.0 32.0 32.0 27.0 32.0 3 29.746725460766125 32.0 32.0 32.0 22.0 32.0 4 28.825127951746577 32.0 27.0 32.0 13.0 32.0 5 29.74004120558979 32.0 32.0 32.0 22.0 32.0 6 33.83822610868207 37.0 37.0 37.0 22.0 37.0 7 33.77974120652204 37.0 37.0 37.0 22.0 37.0 8 33.690878835056445 37.0 37.0 37.0 22.0 37.0 9 33.910060876131524 37.0 37.0 37.0 22.0 37.0 10-11 33.62877445999236 37.0 37.0 37.0 22.0 37.0 12-13 33.55914214063971 37.0 37.0 37.0 22.0 37.0 14-15 34.02494709463302 37.0 37.0 37.0 24.5 37.0 16-17 34.16981923611176 37.0 37.0 37.0 24.5 37.0 18-19 33.7815078262653 37.0 37.0 37.0 22.0 37.0 20-21 34.08184949704942 37.0 37.0 37.0 24.5 37.0 22-23 33.99041410685486 37.0 37.0 37.0 24.5 37.0 24-25 33.462842719568926 37.0 37.0 37.0 22.0 37.0 26-27 33.91181584271025 37.0 37.0 37.0 24.5 37.0 28-29 33.82252230415692 37.0 37.0 37.0 24.5 37.0 30-31 33.44170387910541 37.0 37.0 37.0 22.0 37.0 32-33 33.67095658497021 37.0 37.0 37.0 22.0 37.0 34-35 33.69796628972564 37.0 37.0 37.0 22.0 37.0 36-37 34.2368342547102 37.0 37.0 37.0 24.5 37.0 38-39 34.25054536810016 37.0 37.0 37.0 27.0 37.0 40-41 33.79120792042287 37.0 37.0 37.0 24.5 37.0 42-43 33.62491493189891 37.0 37.0 37.0 22.0 37.0 44-45 33.42250179458733 37.0 37.0 37.0 22.0 37.0 46-47 33.659743910056214 37.0 37.0 37.0 24.5 37.0 48-49 34.02101997818528 37.0 37.0 37.0 24.5 37.0 50-51 34.256469836948924 37.0 37.0 37.0 27.0 37.0 52-53 33.43553469380144 37.0 37.0 37.0 22.0 37.0 54-55 33.51631676097961 37.0 34.5 37.0 24.5 37.0 56-57 33.36850103013974 37.0 34.5 37.0 17.5 37.0 58-59 33.059638565448836 37.0 34.5 37.0 17.5 37.0 60-61 32.891669851864975 37.0 34.5 37.0 13.0 37.0 62-63 32.562728984683076 37.0 32.0 37.0 13.0 37.0 64-65 32.57149915631089 37.0 32.0 37.0 13.0 37.0 66-67 32.61044170154847 37.0 32.0 37.0 13.0 37.0 68-69 32.79571303383146 37.0 32.0 37.0 13.0 37.0 70-71 32.806879096087336 37.0 32.0 37.0 13.0 37.0 72-73 32.79432630725199 37.0 32.0 37.0 13.0 37.0 74-75 32.69676368314579 37.0 32.0 37.0 13.0 37.0 76 32.57878471477714 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.08010590671635498 11101 2 -0.2801775350965734 11101 3 0.6469175703949368 11101 4 0.8101095463317236 11101 5 0.6527682453963379 11101 6 -0.5926381847524027 11101 7 0.4214293067656314 11101 8 0.4591050878471705 11101 9 -0.0835576305015735 11101 10-11 0.38652514760423173 11101 12-13 0.5447865414913196 11101 14-15 0.30334058319908763 11101 16-17 -0.05855911302145955 11101 18-19 0.15692231797431333 11101 20-21 0.3506364352402329 11101 22-23 0.3090944786796612 11101 24-25 -0.32242308002820863 11101 26-27 0.026186031897481143 11101 28-29 0.31659891387867134 11101 30-31 0.41258208766004145 11101 32-33 0.5369892163015351 11101 34-35 0.6112436532424894 11101 36-37 -0.16803693907417028 11101 38-39 -0.1971884697620041 11101 40-41 0.2936097067911305 11101 42-43 0.6750839179940797 11101 44-45 0.1921612871216496 11101 46-47 0.2453335084187387 11101 48-49 0.47149117189081124 11101 50-51 0.47185890901307204 11101 52-53 0.03823884811188094 11101 54-55 0.0514495160517896 11101 56-57 -0.9076941156939284 11101 58-59 0.26173037619657435 11101 60-61 0.6358336190431757 11101 62-63 0.3028825241657742 11101 64-65 -0.938445057826307 11101 66-67 0.05191637109609104 11101 68-69 -0.09681359826582536 11101 70-71 -0.3061815664133718 11101 72-73 -0.17875594322543975 11101 74-75 0.034627332959765056 11101 76 -0.42475770370209176 11102 1 -0.3195276810699035 11102 2 0.07365452597212752 11102 3 0.12661222688348772 11102 4 -0.0596309116835414 11102 5 0.8343865660070264 11102 6 1.2000946396750791 11102 7 0.5198720548572382 11102 8 -0.014238423603210038 11102 9 -0.370275187753478 11102 10-11 0.3576396514210245 11102 12-13 0.36283234301804157 11102 14-15 -0.05662888244975761 11102 16-17 -0.048788120655046896 11102 18-19 0.36779254698195274 11102 20-21 0.3134150611944335 11102 22-23 -0.33695132284706375 11102 24-25 0.5045082176817175 11102 26-27 0.635804352508174 11102 28-29 0.1787973871611186 11102 30-31 -0.19422707264529038 11102 32-33 0.12779837660688287 11102 34-35 0.38357953110508447 11102 36-37 -0.2771056413642441 11102 38-39 0.3156970264212049 11102 40-41 0.3066784090812007 11102 42-43 0.3452823912765197 11102 44-45 0.6432452565873064 11102 46-47 0.0985243481134006 11102 48-49 -0.1718981410862881 11102 50-51 0.15317188611231103 11102 52-53 0.7258113671958526 11102 54-55 0.04180066108995817 11102 56-57 0.12940512094729684 11102 58-59 0.44411205558588307 11102 60-61 0.453543542707294 11102 62-63 0.6922565699672987 11102 64-65 -0.006994676146916845 11102 66-67 -0.9572439342474226 11102 68-69 -0.020905201319266098 11102 70-71 -0.5413571389324616 11102 72-73 0.05767153769058808 11102 74-75 -0.029067323528785494 11102 76 -1.1710783143891135 11103 1 0.5761656082088926 11103 2 0.7685388828138748 11103 3 0.23467876442478897 11103 4 0.8854528299138131 11103 5 0.41909660360529344 11103 6 0.4392424122625229 11103 7 1.0508621425865314 11103 8 1.5448364311307543 11103 9 0.8877856530805204 11103 10-11 0.9299878341713992 11103 12-13 0.6081895265659512 11103 14-15 0.616892822005056 11103 16-17 1.2430826780233133 11103 18-19 0.5146536612578885 11103 20-21 0.4620991218073982 11103 22-23 0.13410940405278637 11103 24-25 1.2073679647479096 11103 26-27 0.664544240852706 11103 28-29 0.7880317496995701 11103 30-31 0.5171492518391574 11103 32-33 -0.31118988817607374 11103 34-35 0.9378406681678584 11103 36-37 0.13097798629895863 11103 38-39 0.2881249630738125 11103 40-41 0.2717291097762029 11103 42-43 0.2530242165015366 11103 44-45 0.6689374065246341 11103 46-47 0.35094544871724764 11103 48-49 0.24256579875648043 11103 50-51 0.07356040155038812 11103 52-53 0.5577015346790475 11103 54-55 -0.0671773496198469 11103 56-57 -0.4036642649476576 11103 58-59 0.896715450823443 11103 60-61 0.4468783951625781 11103 62-63 1.1201362555090526 11103 64-65 0.765137031725935 11103 66-67 -0.40927765875466093 11103 68-69 0.9809177450177557 11103 70-71 0.6959676873179745 11103 72-73 0.15115450453575363 11103 74-75 -0.1557905774879984 11103 76 -0.18708606191104593 11104 1 -0.029135472593988965 11104 2 0.20147763731721824 11104 3 -0.7889444985705794 11104 4 -1.3010628674613791 11104 5 0.8020096247066846 11104 6 0.6567411255924256 11104 7 0.6650155136621834 11104 8 -0.7433776707735191 11104 9 0.163338921222568 11104 10-11 -0.20161278343024946 11104 12-13 -1.325006561956954 11104 14-15 0.054926790095642275 11104 16-17 -0.3613177337111182 11104 18-19 0.4333361110777574 11104 20-21 -0.29019115096666326 11104 22-23 0.7853703407486208 11104 24-25 -1.3309748041661393 11104 26-27 -0.1758829336197607 11104 28-29 0.007357534568328106 11104 30-31 -0.38838342958133154 11104 32-33 -0.0745969905950119 11104 34-35 0.41758848082869093 11104 36-37 -0.07810590459141054 11104 38-39 -0.32849881458959374 11104 40-41 -0.15094201734680013 11104 42-43 0.4255666766147641 11104 44-45 0.020713011259587688 11104 46-47 -0.005976836408848385 11104 48-49 0.24542220637356849 11104 50-51 0.10868649521997042 11104 52-53 -0.4497611518881186 11104 54-55 -0.08220565636200661 11104 56-57 0.37299553947848807 11104 58-59 0.2764889968862292 11104 60-61 0.8592818949052443 11104 62-63 0.4812273517519827 11104 64-65 -0.03223816127458434 11104 66-67 1.182950853854713 11104 68-69 0.289255367251414 11104 70-71 -0.174181566413381 11104 72-73 0.7022095740159386 11104 74-75 -0.5204071497988636 11104 76 -0.3872404623227794 11105 1 0.5746307294113926 11105 2 0.530262181215484 11105 3 -0.33719590478236583 11105 4 0.2321237307288868 11105 5 0.09327888369421089 11105 6 0.711815715956817 11105 7 0.7977129947088955 11105 8 0.1918143077053287 11105 9 -0.5031320985866756 11105 10-11 0.80195777171771 11105 12-13 0.5136092365267828 11105 14-15 0.20819164702888315 11105 16-17 0.5732139366239295 11105 18-19 0.37439749527927546 11105 20-21 0.8482534565168294 11105 22-23 0.19666894676476687 11105 24-25 -0.06562875378707389 11105 26-27 0.2138456063655667 11105 28-29 0.06857054508434857 11105 30-31 -0.2846661392903087 11105 32-33 0.3068899255213964 11105 34-35 0.17958407877440408 11105 36-37 -0.09192346389686179 11105 38-39 -0.4690466257903694 11105 40-41 0.022205451471975834 11105 42-43 -0.21884516002011623 11105 44-45 0.15607618073867968 11105 46-47 0.04709237366697039 11105 48-49 -0.03506201959854849 11105 50-51 -0.012736835667773505 11105 52-53 -0.6802576057888317 11105 54-55 -0.5705646683453693 11105 56-57 0.7976959552280647 11105 58-59 0.10365945421076361 11105 60-61 0.5210818459935282 11105 62-63 -0.13550045455762927 11105 64-65 -0.3269556961241804 11105 66-67 0.24553339237268546 11105 68-69 0.45413675634409856 11105 70-71 0.4173077952887496 11105 72-73 -0.37313892194884346 11105 74-75 0.24325144643493246 11105 76 -1.0775803987375525 11106 1 0.17790182508370123 11106 2 -0.0582455623074587 11106 3 -0.15525930035336089 11106 4 0.8207762129983927 11106 5 0.2167410345119869 11106 6 0.5623005907578005 11106 7 0.5843680822758373 11106 8 -0.151071782901127 11106 9 -0.15648280056959862 11106 10-11 0.48715099794436867 11106 12-13 -0.09624747211411488 11106 14-15 0.3844834403419455 11106 16-17 0.0017129958220749586 11106 18-19 0.15207878055934287 11106 20-21 -0.26985336067814103 11106 22-23 0.17995162153679445 11106 24-25 0.8785020900398166 11106 26-27 0.3480907938022426 11106 28-29 0.12936081864057059 11106 30-31 -0.11669682390457581 11106 32-33 0.20673071290017475 11106 34-35 0.460304877732284 11106 36-37 -0.21263557852995518 11106 38-39 -0.555310918741597 11106 40-41 0.11635800611085756 11106 42-43 -0.16673921125762803 11106 44-45 0.035725912971997786 11106 46-47 0.3971430322282643 11106 48-49 0.27619865488400563 11106 50-51 0.1822262559518535 11106 52-53 0.23051095695542045 11106 54-55 0.24214339360281656 11106 56-57 0.20672765301355867 11106 58-59 0.1671997639516789 11106 60-61 0.4177383809479309 11106 62-63 0.02190293232904139 11106 64-65 1.1244120850308335 11106 66-67 0.6002156908239797 11106 68-69 0.3938530684008441 11106 70-71 0.7802402022941095 11106 72-73 0.1686182064344237 11106 74-75 -0.17916858540758795 11106 76 1.7125484187468842 11107 1 0.10477257338302337 11107 2 0.19515579823675822 11107 3 -0.04641576293839478 11107 4 0.5360539907761677 11107 5 0.20143491206300723 11107 6 0.09730625969203999 11107 7 0.049929306765633896 11107 8 -0.12556157881949304 11107 9 0.6494979250539856 11107 10-11 0.48155292538201167 11107 12-13 -0.30288012517534924 11107 14-15 0.5342850276435342 11107 16-17 -0.3868924463547927 11107 18-19 -0.2425499042479089 11107 20-21 0.14560865746245355 11107 22-23 0.25584447867965565 11107 24-25 0.31904914219401803 11107 26-27 0.9091026985641548 11107 28-29 0.8252933583231155 11107 30-31 -0.3291401345621736 11107 32-33 0.8807669940793161 11107 34-35 -0.14067301342417693 11107 36-37 0.4610463942591565 11107 38-39 -0.3741884697620037 11107 40-41 0.3914430401244573 11107 42-43 -0.06724941533926199 11107 44-45 0.09291128712165175 11107 46-47 0.6025001750854102 11107 48-49 0.6324911718908126 11107 50-51 0.7326089090130736 11107 52-53 0.22384995922299566 11107 54-55 0.4484217382740141 11107 56-57 0.2530003287505167 11107 58-59 0.42081370952990227 11107 60-61 -0.13236082540127114 11107 62-63 0.8806047463879949 11107 64-65 -0.4837228356040839 11107 66-67 0.1123330377627525 11107 68-69 0.06746417951195127 11107 70-71 -0.1561260108578182 11107 72-73 0.40685516788567355 11107 74-75 -0.03659488926246013 11107 76 1.7736311851867939 11108 1 -0.3323022565489495 11108 2 0.4655639615020668 11108 3 0.04882233229970012 11108 4 0.1541095463317248 11108 5 -0.5043474008621658 11108 6 0.48747065878501417 11108 7 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-0.13669354071617335 21612 4 -0.31116823144605377 21612 5 -0.22217619904810348 21612 6 -0.27769374030796 21612 7 -0.8250706932343661 21612 8 -1.500561578819493 21612 9 -0.3921687416126858 21612 10-11 -0.7927526301735455 21612 12-13 -0.19871345850867783 21612 14-15 -0.53863163902313 21612 16-17 -0.6160591130214641 21612 18-19 -0.6001887931368017 21612 20-21 -0.35439134253754645 21612 22-23 -0.5740166324314586 21612 24-25 -0.25386752447265337 21612 26-27 -0.7020084125469594 21612 28-29 -0.12262330834354884 21612 30-31 -0.8603901345621736 21612 32-33 -0.9213163392540196 21612 34-35 -1.3073396800908412 21612 36-37 -0.6743702724075007 21612 38-39 -0.17974402531756084 21612 40-41 -0.9592514043199856 21612 42-43 -0.7339160820059263 21612 44-45 -0.6327831573227911 21612 46-47 -0.2829164915812612 21612 48-49 -0.6730643836647445 21612 50-51 -0.37850220209804064 21612 52-53 0.08496107033410993 21612 54-55 -0.5481060395037645 21612 56-57 -0.6567218934717047 21612 58-59 0.11525815397434513 21612 60-61 -0.6531941587346068 21612 62-63 -0.40758969805644796 21612 64-65 -0.8240006133818625 21612 66-67 -0.8216947400150261 21612 68-69 -0.14086915382138443 21612 70-71 -1.1005704553022646 21612 72-73 -0.9716170543365514 21612 74-75 -1.5574282225957958 21612 76 -0.9902577037020919 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 27.0 12 71.0 13 108.0 14 177.0 15 290.0 16 497.0 17 776.0 18 972.0 19 1018.0 20 1246.0 21 1562.0 22 2050.0 23 2562.0 24 3072.0 25 3504.0 26 3852.0 27 4388.0 28 4823.0 29 5398.0 30 6613.0 31 8327.0 32 10737.0 33 16435.0 34 29481.0 35 54631.0 36 51913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.869009108113396 16.000727157466883 8.225735780808636 22.90452795361108 2 37.900154304441266 17.051806201080595 26.850837486189523 18.197202008288617 3 35.70203324414778 18.971351860311188 25.678447239132257 19.648167656408773 4 32.61114788331919 17.682511862921498 26.964956603615274 22.741383650144034 5 32.3701604407693 25.834133517297957 29.324023231748814 12.471682810183934 6 42.92559687508739 30.249284495697655 14.597686147650254 12.227432481564694 7 42.16254766144294 23.71931721778366 16.289259511312892 17.828875609460503 8 40.51758695591375 28.32045270213579 15.340225791715998 15.821734550234462 9 39.23107759143073 15.380778804292095 15.799826600911743 29.588317003365432 10-11 40.6508991581754 21.316201627714022 21.32645641250338 16.706442801607206 12-13 40.032582248035276 19.20534740414107 20.964742185387863 19.79732816243579 14-15 40.856927107125216 20.58042081907763 17.798577381673766 20.764074692123394 16-17 40.65952249993008 21.59844127271202 18.451154595541965 19.290881631815935 18-19 38.28533604318358 21.39554179912924 21.10163802988915 19.21748412779803 20-21 40.075840385587995 21.237172510074373 19.74157400264299 18.945413101694637 22-23 40.53311394104376 21.11689631383663 19.174761807096377 19.17522793802323 24-25 39.126826880492295 22.971725600801847 19.070184844176126 18.831262674529732 26-27 40.10614419668142 22.625915126596883 19.920100503683813 17.34784017303788 28-29 40.40796061648626 21.499398618220468 20.069368688987723 18.023272076305556 30-31 38.70172919395971 22.826152834824445 21.563873879334057 16.908244091881784 32-33 40.14471604604432 23.459711220622037 19.66860771414851 16.726965019185133 34-35 39.15849004701993 24.153139985127062 20.187100639443127 16.50126932840988 36-37 40.762188511920996 21.099011770630074 21.72519343791453 16.413606279534395 38-39 40.485115509243066 20.798191015688744 22.192694127794486 16.5239993472737 40-41 40.32629903052031 21.57401169716056 21.096381526038886 17.003307746280242 42-43 38.5779182487128 21.704391037519258 22.32135989539266 17.396330818375276 44-45 37.96159045354962 20.37220901505617 22.737146319862024 18.929054211532186 46-47 39.65286178100039 19.355507853378736 21.571561422767925 19.420068942852946 48-49 39.78250533714935 19.492481378243077 23.404448712092254 17.320564572515313 50-51 41.198597891243345 19.47034036562969 21.878816411384676 17.45224533174229 52-53 37.509205999981354 20.151118237668623 23.024089421723364 19.315586340626663 54-55 38.58501340170143 20.459620090898497 21.371635007574874 19.5837314998252 56-57 38.50566139761238 20.466103867650556 21.084219407709195 19.944015327027873 58-59 37.14679258299384 21.64388861439212 22.813400206960203 18.395918595653836 60-61 36.81374514063039 20.843549274240914 22.86700476381366 19.475700821315037 62-63 36.886560262529834 22.207823687350835 22.548338678400956 18.35727737171838 64-65 37.13537248175115 22.30252547381767 22.301127094073667 18.260974950357518 66-67 37.59264265804021 23.90576785031743 19.809447453550487 18.692142038091863 68-69 37.46608929120792 23.93047255912816 19.42862203660026 19.174816113063663 70-71 38.16040946085789 23.64398720900963 19.453586045514296 18.742017284618182 72-73 38.826872288797155 23.215238912185583 19.05843028202337 18.899458516993896 74-75 40.80588386134229 22.032309951744878 18.70107466722615 18.46073151968669 76 43.1284879736301 19.804463673789087 18.556835612414737 18.51021274016607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 1.0 5 2.5 6 3.0 7 3.0 8 4.0 9 5.0 10 7.0 11 11.5 12 15.0 13 13.0 14 16.5 15 30.5 16 47.5 17 56.0 18 72.5 19 95.5 20 124.0 21 146.0 22 188.5 23 282.0 24 354.5 25 376.0 26 560.0 27 912.0 28 1191.5 29 1303.0 30 1514.0 31 1967.0 32 2599.5 33 2990.0 34 3306.0 35 4100.0 36 4956.5 37 5335.0 38 5876.0 39 7250.5 40 8693.0 41 9607.0 42 9912.0 43 11165.0 44 14106.0 45 13584.5 46 11375.0 47 11315.0 48 10455.0 49 9214.5 50 8774.0 51 8057.5 52 6430.0 53 5178.0 54 4837.0 55 4570.5 56 3729.0 57 2738.0 58 2322.0 59 1995.5 60 1493.5 61 1175.5 62 1033.0 63 965.0 64 841.5 65 724.5 66 636.5 67 610.0 68 578.0 69 524.0 70 463.0 71 424.0 72 398.5 73 373.0 74 341.0 75 309.0 76 327.0 77 325.0 78 288.5 79 272.0 80 241.5 81 204.5 82 195.5 83 193.0 84 188.5 85 175.5 86 181.5 87 196.0 88 179.0 89 191.5 90 214.5 91 215.5 92 223.0 93 260.5 94 356.5 95 478.5 96 542.0 97 647.5 98 1149.0 99 22321.5 100 43098.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010720911370691826 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0037290126506754175 20-21 0.002097569616004922 22-23 9.322531626688544E-4 24-25 0.01351767085869839 26-27 0.006758835429349195 28-29 0.01305154427736396 30-31 0.019111189834711516 32-33 0.020975696160049222 34-35 0.023539392357388573 36-37 0.02773453158939842 38-39 0.022840202485386932 40-41 0.017479746800041017 42-43 0.008157215173352475 44-45 0.0018645063253377087 46-47 0.003962075941342631 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.004195139232009844 56-57 0.005127392394678699 58-59 0.0 60-61 0.0 62-63 0.002796759488006563 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0037290126506754175 72-73 0.01421686073070003 74-75 0.022840202485386932 76 0.021907949322718077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 214534.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.246170770134334 #Duplication Level Percentage of deduplicated Percentage of total 1 67.90500007227627 21.89676228476605 2 8.04723976929415 5.189853356577513 3 6.2345509475418845 6.031211835886154 4 4.838173434134636 6.240502670905311 5 3.5993581867329683 5.803275937613618 6 2.4501655126555746 4.740507332171124 7 1.5496032032842337 3.497813866333541 8 1.1520837248297893 2.972023082588308 9 0.7401089926133653 2.14791128678904 >10 3.4403503953511905 17.579031761865252 >50 0.03324708365255352 0.6907995935376211 >100 0.0043365761285939376 0.33980627779279743 >500 0.002891050752395958 0.5206633913505552 >1k 0.0 0.0 >5k 0.001445525376197979 2.5604333112700086 >10k+ 0.001445525376197979 19.789404010553106 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 42455 19.789404010553106 No Hit CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG 5493 2.5604333112700086 No Hit ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC 608 0.28340496145133176 No Hit TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT 509 0.2372584298992234 No Hit CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG 419 0.195307037579125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002330632906672136 0.0 2 0.0 0.0 0.0 0.002330632906672136 0.0 3 0.0 0.0 0.0 0.002330632906672136 0.0 4 0.0 0.0 0.0 0.0037290126506754175 0.0 5 0.0 0.0 0.0 0.004661265813344272 0.0 6 0.0 0.0 0.0 0.005127392394678699 0.0 7 0.0 0.0 0.0 0.006059645557347553 0.0 8 0.0 0.0 0.0 0.006059645557347553 0.0 9 0.0 0.0 0.0 0.00978865820802297 0.0 10 0.0 0.0 0.0 0.010254784789357398 0.0 11 0.0 0.0 0.0 0.011187037952026252 0.0 12 0.0 0.0 0.0 0.011187037952026252 0.0 13 0.0 0.0 0.0 0.011187037952026252 0.0 14 0.0 0.0 0.0 0.011653164533360679 0.0 15 0.0 0.0 0.0 0.013517670858698387 0.0 16 0.0 0.0 0.0 0.013983797440032815 0.0 17 0.0 0.0 0.0 0.014449924021367242 0.0 18 0.0 0.0 0.0 0.01491605060270167 0.0 19 0.0 0.0 0.0 0.015382177184036097 0.0 20 0.0 0.0 0.0 0.01771281009070823 0.0 21 0.0 0.0 0.0 0.02144182274138365 0.0 22 0.0 0.0 0.0 0.023772455648055787 0.0 23 0.0 0.0 0.0 0.024238582229390213 0.0 24 0.0 0.0 0.0 0.025636961973393494 0.0 25 0.0 0.0 0.0 0.02656921513606235 0.0 26 0.0 0.0 0.0 0.02843372146140006 0.0 27 0.0 0.0 0.0 0.05034167078411814 0.0 28 0.0 0.0 0.0 0.05733356950413454 0.0 29 0.0 0.0 0.0 0.08809792387220673 0.0 30 0.0 0.0 0.0 0.11187037952026252 0.0 31 0.0 0.0 0.0 0.15335564525902654 0.0 32 0.0 0.0 0.0 0.19623929074179383 0.0 33 0.0 0.0 0.0 0.2605647589659448 0.0 34 0.0 0.0 0.0 0.3519255689074925 0.0 35 0.0 0.0 0.0 0.476847492705119 0.0 36 0.0 0.0 0.0 0.6353305303588243 0.0 37 0.0 0.0 0.0 0.9387789348075364 0.0 38 0.0 0.0 0.0 1.2534143772082746 0.0 39 0.0 0.0 0.0 1.6626735156199017 0.0 40 0.0 0.0 0.0 2.121342071652978 0.0 41 0.0 0.0 0.0 2.717984095761045 0.0 42 0.0 0.0 0.0 3.434886777853394 0.0 43 0.0 0.0 0.0 4.162510371316435 0.0 44 0.0 0.0 0.0 4.9148386735902 0.0 45 0.0 0.0 0.0 5.550635330530358 0.0 46 0.0 0.0 0.0 6.266139632878705 0.0 47 0.0 0.0 0.0 6.905665302469538 0.0 48 0.0 0.0 0.0 7.529808794876336 0.0 49 0.0 0.0 0.0 8.148824894888456 0.0 50 0.0 0.0 0.0 8.69465912163107 0.0 51 0.0 0.0 0.0 9.319734867200538 0.0 52 0.0 0.0 0.0 10.015661853132837 0.0 53 0.0 0.0 0.0 10.691545396067756 0.0 54 0.0 0.0 0.0 11.417770609786794 0.0 55 0.0 0.0 0.0 12.118824988113772 0.0 56 0.0 0.0 0.0 12.70707673375782 0.0 57 0.0 0.0 0.0 13.29998974521521 0.0 58 0.0 0.0 0.0 13.864002908629868 0.0 59 0.0 0.0 0.0 14.41076938853515 0.0 60 0.0 0.0 0.0 14.897405539448293 0.0 61 0.0 0.0 0.0 15.354209589156032 0.0 62 0.0 0.0 0.0 15.89631480324797 0.0 63 0.0 0.0 0.0 16.434224878107898 0.0 64 0.0 0.0 0.0 17.04811358572534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTTA 15 0.0022210511 70.01096 13 TACCGTT 15 0.0022210511 70.01096 12 GAGCTCG 15 0.002223106 69.994644 41 ACATGTT 15 0.002223106 69.994644 32 TGTGCGC 25 2.8344075E-6 69.99464 3 TGCGCGT 25 2.8344075E-6 69.99464 5 GCGTGTG 20 7.9169484E-5 69.99464 8 ACGGTTA 25 2.3847942E-4 56.008766 50 TGTCGGT 25 2.387544E-4 55.99571 43 TACTCGC 20 0.006940069 52.508217 20 TCGTTGT 20 0.0069464725 52.49598 34 TTTAACG 20 0.0069464725 52.49598 37 CGACCGG 20 0.0069464725 52.49598 30 TAACGGC 20 0.0069464725 52.49598 39 GTGTCGG 20 0.0069464725 52.49598 42 TTAACGG 20 0.0069464725 52.49598 38 GATCTAC 20 0.0069464725 52.49598 1 CGCGTCT 40 7.45953E-7 52.49598 7 CGCGTCG 20 0.0069464725 52.49598 7 CGGTACC 20 0.0069464725 52.49598 48 >>END_MODULE