FastQCFastQC Report
Wed 25 May 2016
SRR1780935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214534
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT187708.749195931647199No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90424.214716548425891No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT48092.2416027296372603No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG34821.6230527562064754No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT9990.46566045475309276RNA PCR Primer, Index 30 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC7230.33700951830479087No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6990.3258224803527646No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT4030.18784901227777415No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT3670.17106845534973478No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC3590.16733944269905937No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT3570.1664071895363905No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT2560.11932840482161336No Hit
CTATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC2500.1165316453336068No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT2210.1030139744749084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA252.8339018E-669.9967439
TTCCGTC207.915773E-569.99673545
ATTCCGT207.915773E-569.99673544
ACGGATA207.915773E-569.99673533
CGTACAG150.00222284269.99673528
CGTAAGA207.915773E-569.99673541
ACGTATG501.4551915E-1162.99706640
AGGGGGG33300.061.37851770
CGTATGA2200.058.86089341
TCGTATT308.359282E-658.33061240
CTTAAGC553.6379788E-1157.27005851
CGTCTGC252.3871896E-455.9973951
CCGTCGT252.3871896E-455.9973947
AACTCGT509.767973E-1055.9973937
CACGTAT509.767973E-1055.9973939
CGAATGC700.054.99743741
AAGCTTG700.054.99743754
ATTATAC11050.054.4898031
ACGTCAT452.690831E-854.44190647
ATTGACG1350.054.44190629
Produced by FastQC (version 0.11.5)