FastQCFastQC Report
Wed 25 May 2016
SRR1780934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915492
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463745.0654729915717445No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG52870.577503681080774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC10250.046.77300649
GCCGTAT9600.046.66684754
TCGCCGT10000.045.50017552
GTAGATC10550.044.1330438
GTCGCCG10800.044.07424551
TCGGTGG10900.043.66035545
CGCCGTA10350.043.6233653
CTCGGTG11100.043.19128844
CGTATCA10600.042.25969756
TCTCGGT12000.041.12066743
GATCTCG11250.041.06682641
CGGTGGT11600.041.02567746
AGATCTC11750.040.21512240
CCGTATC11100.040.04520455
ATCTCGG11650.039.95505542
GTGGTCG12050.039.7818248
GGTGGTC12450.038.78685447
TAGATCT12050.038.63718839
GTATCAT11850.038.38628857
GGTCGCC12750.037.3334850