FastQCFastQC Report
Wed 25 May 2016
SRR1780934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915492
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT243222.656713548561866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84230.9200517317464271No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG42110.45997125043146203No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT36680.4006588806892906No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT15980.17455095183791886Illumina PCR Primer Index 10 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC14240.15554477810838324No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC9460.1033324157939119No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG43700.055.58200570
GCCGTCT47400.049.18124846
CCGTCTT47150.048.92235647
CGTCTTC48200.048.7280648
TATGCCG49950.048.4197443
TGCCGTC49650.048.00735545
CGTATGC50750.047.93496341
ATGCCGT50250.047.85205544
TCGTATG53000.047.68575740
CTCGTAT52350.047.6760439
GTATGCC50850.047.6341942
ATCTCGT53600.047.0866537
TCTCGTA53600.046.9560438
AAGGGGG51700.046.91361269
CTTGAAA52300.046.44486257
TATCTCG54550.045.9457836
CTGCTTG48800.045.8311754
GTCTTCT49500.045.6805149
TCTTCTG49550.045.6294350
CTTCTGC50050.044.8938851