FastQCFastQC Report
Wed 25 May 2016
SRR1780933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310099
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4090613.191271174689373No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG56971.8371552310713677No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC6960.2244444516106147No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT5790.18671456534848546No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG4040.13028097478547174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.002223794369.996611
GGTCTAA150.002223794369.996611
CGCGTCT308.376128E-658.3211067
TGTCTTC252.3850703E-456.01535832
CAGGTAT252.3888763E-455.9972881
TGTGCGC252.3907812E-455.988263
GCCTTGA200.00694860252.497461
GACACCG200.00694860252.497461
GCACGTG200.00694860252.4974651
GACCTAT200.00694860252.497461
GGAAACT200.00695303652.48899547
CTTTATA352.0845124E-549.997582
GCGCGTC352.0865025E-549.9895176
TGCGCGT352.0865025E-549.9895175
GCCGTAT8450.048.04501354
TCGCCGT8650.047.74335552
TGGTCGC8650.047.73565749
GTCGCCG8700.047.46896751
ATCTCGG8350.046.94383642
TCGGTGG8650.046.9265845
Produced by FastQC (version 0.11.5)