FastQCFastQC Report
Wed 25 May 2016
SRR1780933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310099
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT224697.245750550630604No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94023.0319349627054586No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG37511.2096137040106547No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT31041.0009706577576838No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC13090.42212325741134277No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT13060.42115582443026256RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC7290.23508621440249725No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4870.15704662059535826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT150.002222372770.00790437
CAACTCG150.002222372770.00790436
CGAATGC401.3460522E-1070.007941
GGTCATA150.00222379569.996611
ACGAGAT1050.063.34048527
GAGACGA1000.062.9969524
TGACGTC950.062.63865345
AGGGGGG36850.061.06896270
CCGACTT352.966517E-760.0067847
CGACTTC352.966517E-760.0067848
AGATAGC1000.059.50671430
CACGTAT308.36013E-658.3399239
ATCTGCA252.3888767E-455.99728852
ACGTATG452.69265E-854.45059640
CGTATGA1050.053.33935541
TGCCGAC608.367351E-1152.50592845
TCCTGAC200.006944172552.50592442
CGTCTAC200.006944172552.50592448
AAGCCGT800.052.50592444
GGTACGG200.00694860452.497461