FastQCFastQC Report
Wed 25 May 2016
SRR1780932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432469
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5146611.900506163447554No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG63921.4780250145097105No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT7120.16463607796165736No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5800.13411365901370964No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC5490.12694551516987346No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAG150.002226091569.9831212
CCGAGCG200.00694303652.51162741
CGCACTT200.00694303652.51162743
ACGTGGA200.006955750352.4873449
TCGCCGT9600.046.2909252
GTCGCCG9700.046.17442751
CTCGGTG9850.045.84771344
GCCGTAT9550.045.8004754
CGGTGGT9950.045.7281946
TGGTCGC9800.045.70326649
TCGGTGG10000.045.51007545
AGATCTC10100.044.00946440
ATCTCGG10150.043.802842
GATCTCG10150.043.802841
CGTATCA10000.043.7394556
CGCCGTA10150.043.43779853
TCTCGGT10350.043.2946143
GTAGATC10300.043.16489438
GTATCAT10350.042.93650457
GGTGGTC10750.042.32516547