FastQCFastQC Report
Wed 25 May 2016
SRR1780932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432469
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT207634.8010377622442295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101582.348838876312522No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT91702.1203831950960645No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG38520.8906996802082923No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT15630.36141318799728994TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC9330.21573800665481224No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6190.14313164643014875No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4880.1128404579287764TruSeq Adapter, Index 12 (95% over 21bp)
CTATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC4590.1061347749780909No Hit
TATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG4490.1038224705123373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA150.002224371569.9967628
CAGTGGA207.924851E-569.9967627
TGCCGAC550.063.63342345
AGGGGGG43650.061.5850370
GCCGACT700.060.011146
CGACTTC700.060.011148
CCGACTT601.8189894E-1258.34412847
CTCGAAA252.3885448E-456.00388739
ATCGTAT1400.055.00381539
CACGTAT653.6379788E-1253.8498939
CGTCTTA850.053.53931848
CGTATGA2650.052.82774441
AACGAAG200.00695039252.4975729
ACGTATG750.051.3368940
ATATCGT1300.051.15739437
TGACGTC2400.051.03930745
AGCGTCA552.2828317E-950.906743
GCCGTCT51250.050.7508546
CCGTCTT50200.050.69663247
CGTCTTC51450.050.68964848