FastQCFastQC Report
Wed 25 May 2016
SRR1780931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205447
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3584417.446835436876665No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG51512.5072159729759984No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC6210.30226773815144536No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT5480.2667354597536104No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG4280.20832623499004607No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2270.11049078351107586No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCG150.002222296870.023
TAGACCC150.002222296870.062
TGTGGAC150.002222296870.020
TGGAACC150.002222296870.02
TGTGCGC207.913148E-570.03
GGGCGCT150.002222296870.013
AGCCGGC200.006943951452.563
ACAGTAG200.006943951452.511
CCGGGCT200.006943951452.527
GTCTGCT200.006943951452.510
CGTGCTC200.006943951452.552
ACGCATT200.006943951452.534
TGGACAT200.006943951452.539
CACTGGC200.006943951452.511
GTGGACA200.006943951452.521
GGGCACT200.006943951452.58
AAGATCG200.006943951452.551
CACGTCT200.006943951452.57
CACCGGG200.006943951452.525
AGGGCAC200.006943951452.57
Produced by FastQC (version 0.11.5)