FastQCFastQC Report
Wed 25 May 2016
SRR1780931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205447
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT194079.446231874887442TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86744.2220134633263084No Hit
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT32461.579969529854415No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG30281.4738594382006065TruSeq Adapter, Index 5 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT12050.5865259653341252TruSeq Adapter, Index 5 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC10950.5329841759675245RNA PCR Primer, Index 5 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC5610.2730631257696632RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3510.17084698243342564TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT2460.11973891076530686No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT2350.11438473182864681No Hit
CTATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2190.10659683519350488TruSeq Adapter, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG150.00222018270.01681
CTTAAGC150.002222327569.99975651
CAGCGCC150.002222327569.99975629
AGATCTC550.069.99975635
ACGCCGA207.9132835E-569.99975610
GGTGTCC150.002222327569.99975640
TCCGACG150.002222327569.99975653
GCTATAC150.002222327569.9997563
TCAGCAT150.002222327569.99975653
CGAATGC401.3460522E-1069.99975641
GACGGCA150.002222327569.99975656
AACTCGT252.8327468E-669.99975637
CCCGGTG207.9132835E-569.99975633
AAGCTTG207.9132835E-569.99975654
TGTCCAG150.002222327569.99975625
AGCCGTC800.065.6247745
AAGCCGT850.061.76449244
AGGGGGG32000.060.70291570
TAAGCCG900.058.33313443
CAATTCG252.3864416E-455.9998059
Produced by FastQC (version 0.11.5)