FastQCFastQC Report
Wed 25 May 2016
SRR1780930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158009
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2941718.61729395161035No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG1670.10569018220481113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT207.889783E-570.0316854
CTGTTCG207.914584E-569.9873359
TGCCGGC150.002222937369.98733519
CGGTGGT308.340088E-658.3412546
GTTTAGC252.3793771E-456.025341
GATCTCG252.3793771E-456.0253441
TTGAAAG200.00693726852.5071223
ACCTAGG200.00694596352.49050523
GTTCGAA200.00694596352.49050511
TGTTCGA200.00694596352.49050510
TGTAACG200.00694596352.49050512
TCGCCGT305.850856E-446.68778652
CTCGGTG305.850856E-446.68778644
CGCCGTA305.850856E-446.68778653
CGTATCA451.671282E-646.68778256
TCGGTGG451.6749345E-646.67299745
CCATGTA305.8691594E-446.6582269
TGGTCGC305.8691594E-446.65822649
ATCTCGG350.001249698240.018142
AGATCTC350.00125164740.0054340
Produced by FastQC (version 0.11.5)