FastQCFastQC Report
Wed 25 May 2016
SRR1780930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158009
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT1805811.428462935655565No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG23451.4840926782651622No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC11510.7284395192678897No Hit
CATATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT11200.7088203836490327No Hit
CTTATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCGT4940.31264041921662694No Hit
ATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTCTT3920.24808713427716145TruSeq Adapter, Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC3710.23479675208374207No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3120.1974571068736591No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT2650.16771196577410147No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT2410.15252295755305076No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAGCGATCTCGTATGCCGT2330.14745995481270055No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGCTCTCGTATGCCGT1720.10885455891753001No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAT150.00222010770.0098139
AACGGGG150.00222010770.0098168
TACGCGA301.0198164E-769.98765631
TGTCCGA353.6907295E-969.98765629
AGGGGGG24550.064.7341270
GCGATCA550.063.6050134
CGAATGC501.4551915E-1163.0088441
CGAGATC453.8380676E-1062.19156333
CGTCTTA401.0606527E-861.23919748
CCGAGAT401.0606527E-861.23919732
GACCACG700.059.98941818
CGAGACC601.8189894E-1258.3415115
CGTCTAC800.056.86496748
TCGTATT252.3830523E-456.00785440
AACTCGT252.3830523E-456.00785437
CAGTCCG750.056.00785428
CACGTAT509.713403E-1056.00785439
AGCGATC750.055.9724133
ATATCGT950.055.27091237
CGCTCTC950.055.2534135
Produced by FastQC (version 0.11.5)