FastQCFastQC Report
Wed 25 May 2016
SRR1780929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266351
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3555013.347049569928402No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG150.002223519669.99605643
GGTACAT308.365294E-658.3300481
GATCTCG553.6379788E-1157.30177341
AGATCTC608.367351E-1152.52662740
GTCGCGG200.00694775152.49704442
ACCCGTG200.00694775152.4970449
ATCTCGG608.367351E-1152.4970442
GTCGCCG608.367351E-1152.4970451
TCGGTGG608.367351E-1152.4970445
TCGCCGT651.8553692E-1048.4588152
CTCGGTG651.8553692E-1048.4588144
GCCGTAT651.8553692E-1048.4588154
TGGTCGC651.8553692E-1048.4588149
CGGTGGT703.8198777E-1044.99746346
TAGATCT855.638867E-1141.19735339
GCTGGAT350.001250909340.02028336
ATCCCAT350.001254380239.99774664
CGGAGCT459.185918E-538.886749
GTAGATC951.8553692E-1036.8607938
ATCGCCG501.7018821E-435.0177535
Produced by FastQC (version 0.11.5)