FastQCFastQC Report
Wed 25 May 2016
SRR1780929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266351
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT218568.205713513371453No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG27281.0242124114420446No Hit
CATATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT13580.5098535391269415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC13530.5079763169652075No Hit
CTTATACACATCTCCGCGCCCACGAGACACGTGACGATCTCGTATGCCGT6380.23953354783725236No Hit
ATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTCTT4730.1775852165000319Illumina PCR Primer Index 1 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC4000.1501777729387162No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3930.1475496619122887No Hit
CTAATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT2930.11000521867760961No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGCTCTCGTATGCCGT2790.10474899662475455No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGCCGATCTCGTATGCCGT2730.10249633003067381No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCA150.002221391370.012961
CGACACA150.002223046369.9998223
AAACGTG252.835046E-669.9998227
CGCTCAA150.002223046369.9998226
GAAACGT252.835046E-669.9998226
AGCCGTC207.91756E-569.9998145
ACGAACT401.3460522E-1069.9998134
AGGGGGG29050.064.216770
AACTCGT600.064.166537
TGACGAA800.061.24983632
ACGTGCC650.059.2306129
CGAACTC601.8189894E-1258.3331835
CCGATCT601.8189894E-1258.3331834
CGTCTTA308.362633E-658.3331848
ACGTATG601.8189894E-1258.3331840
ACTACTT252.3877235E-455.9998519
CACGTAT509.767973E-1055.9998539
TATGCCG37300.055.6432843
CCGTCTT35750.055.60824247
CGTATGC37750.055.53627841
Produced by FastQC (version 0.11.5)