FastQCFastQC Report
Wed 25 May 2016
SRR1780928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155013
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3453822.28071194028888No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG2400.15482572429409147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC207.907031E-570.01
GCCGTAT550.063.6363654
GTCGCCG650.059.23076651
CGTATCA601.8189894E-1258.33333256
GCGCGTC308.34608E-658.3333326
GTCTCGG308.34608E-658.33333242
TGCGCGT308.34608E-658.3333325
GTGCGCG308.34608E-658.3333324
CTCGGTG750.056.00000444
TCTCGGT750.056.00000443
CGCGTCT252.3845669E-455.9999967
TCGCCGT700.055.00000452
ATCACGC200.00694075352.553
GGACCCC200.00694075352.543
TGCCGGC407.4420313E-752.519
CGGTGGT950.051.5789546
TGGCGCT552.2646418E-950.9090913
GTAGATC552.2646418E-950.9090938
GATCTCG552.2646418E-950.9090941
GGTCTCG352.0790469E-550.00000441
Produced by FastQC (version 0.11.5)