FastQCFastQC Report
Wed 25 May 2016
SRR1780928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155013
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT2056213.264693928896287No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG26151.686955287621038No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC13480.8696044847851472No Hit
CATATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT11370.7334868688432583No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT6020.3883545251043461Illumina PCR Primer Index 4 (95% over 22bp)
CTTATACACATCTCCGCGCCCACGAGACTTCTACCAATCTCGTATGCCGT5620.3625502377219975No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC5430.35029320121538193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4490.28965312586686276No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT2530.16321211769335475No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCACTCTCGTATGCCGT2340.15095508118673917No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATATCGTATGCCGT2330.15030997400218046No Hit
CTAATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT2260.14579422371026945No Hit
CTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTC1970.12708611535806674No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGACGT1640.10579757826762917No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA150.00222126570.03
AGCCGAC150.00222126570.045
AAACGGG150.00222126570.068
AGGGGGG29550.063.8409570
CCGACTT700.060.00000447
AGCTTGA1000.055.99999655
TGACGAT252.3845669E-455.99999612
AAAAACG252.3845669E-455.99999666
GACGATA252.3845669E-455.99999613
GTGACGA252.3845669E-455.99999611
ATATCGT1200.055.41666437
TCAGCTT950.055.2631653
CGACTTC700.055.00000448
TGCCGAC653.6379788E-1253.84615345
GCCGACT850.053.5294146
CGTCTAC407.4420313E-752.548
GACCACG200.00694075352.518
TCTTTAT1200.052.51
CTTTATA1200.052.52
AATCTCG38100.051.90288536
Produced by FastQC (version 0.11.5)