FastQCFastQC Report
Wed 25 May 2016
SRR1780927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57755
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3846766.6037572504545No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG2780.48134360661414594No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980.16968227859059823No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG940.16275647130118603No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG650.11254436845294778No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG600.10388710934118259No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590.10215565751882953No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGC150.00221571269.987883
GTATCAT301.0083568E-769.9878857
ATGCGCA150.00221571269.987884
CGTATCA353.6361598E-969.9878856
TATCATT301.0083568E-769.9878858
CGGCTGG207.8718775E-569.987889
GTTGGGG207.8718775E-569.987881
TGGCGGT150.00221571269.9878813
GCCGTAT353.6361598E-969.9878854
TTGTGCG207.8718775E-569.987882
TGTGCGG150.00221571269.987883
CGCCGGG150.00221571269.9878853
CATGTGG150.00221571269.987889
TCTCGGT501.2732926E-1162.9890943
GGTCGCC501.2732926E-1162.9890950
CCGTATC401.0450094E-861.2393955
CATTAAA401.0450094E-861.2393961
ATCATTA401.0450094E-861.2393959
TGGCGCT401.0450094E-861.2393913
TCATTAA401.0450094E-861.2393960