FastQCFastQC Report
Wed 25 May 2016
SRR1780927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57755
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT2379241.1947017574236No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG31605.471387758635616No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT13632.359968833867198No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC12712.2006752662107174No Hit
CTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT6521.128906588174184No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC5360.928058176781231No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4920.8518742965976971No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT4710.8155138083282834RNA PCR Primer, Index 15 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT3020.522898450350619No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATATCGTATGCCGT2960.5125097394165007No Hit
CTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT2920.5055839321270885No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAGCCAATCTCGTATGCCGT2170.37572504545061036No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGACGT1950.3376331053588434No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGACAATCTCGTATGCCGT1890.32724439442472514No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCATCTCGTATGCCGTC1690.2926153579776643No Hit
CTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC1600.2770322915764869No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCAGT1380.23894035148471993No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG1300.22508873690589556No Hit
CTTATACACATCTCCGAGCCCACGAGCCGCTTGCCAATCTCGTATGCCGT1280.2216258332611895No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCAAATCTCGTATGCCGT1280.2216258332611895No Hit
ACTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG1260.2181629296164834No Hit
TCTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCG1250.21643147779413038No Hit
CTTATACACATCACCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1100.19045970045883473No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG1040.18007098952471648No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGA1010.17487663405765733No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC950.16448792312353908No Hit
TATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCT910.1575621158341269RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCACTCTCGTATGCCGT900.15583066401177387No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC810.14024759761059646No Hit
CTTATACACATCTCCGAGCCAACGAGACGCTTGCCAATCTCGTATGCCGT780.13505324214353737No Hit
CATATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT770.1333217903211843No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGCCAATCTCGTATGCCGT760.13159033849883128No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCTATCTCGTATGCCGT750.1298588866764782No Hit
CTTATACACATCTCCGAGACCACGAGACGCTTGCCAATCTCGTATGCCGT740.12812743485412517No Hit
TCATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG740.12812743485412517No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGAATGCCGT730.12639598303177213No Hit
CATAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT710.12293307938706605No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAAACTCGTATGCCGT700.12120162756471302No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC670.11600727209765388No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG640.11081291663059475No Hit
CTTATACACATCTCCGAGCCCACGAGACGATTGCCAATCTCGTATGCCGT630.1090814648082417No Hit
CTTATACACATCTCCGAGCACACGAGACGCTTGCCAATCTCGTATGCCGT620.10735001298588867No Hit
CAAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT610.10561856116353562No Hit
TTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC590.10215565751882953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTA600.070.04072638
GAGAAGC207.842448E-570.04072624
TCTAGTA207.842448E-570.04072638
TATCGAA150.002209079570.04072638
ACGTATG252.79724E-670.04072640
GAATGAC150.002209079570.04072642
AGTATGC207.842448E-570.04072641
TAGTATG252.79724E-670.04072640
ACGAGAA150.002209079570.04072622
AGACGAT301.0030817E-770.04072625
CGAATGA150.002209079570.04072641
GACGATT252.79724E-670.04072626
CTAGTAT207.842448E-570.04072639
CGAGAAG150.002209079570.04072623
AATGACG150.002209079570.04072643
GAGACGA353.614332E-970.04072624
ATCTAGT207.8762205E-569.9800937
AACTCGT550.069.9800937
CAGAGCC252.8116992E-669.9800914
CTCAGAG207.8762205E-569.9800912
Produced by FastQC (version 0.11.5)