FastQCFastQC Report
Wed 25 May 2016
SRR1780926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246387
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3865615.689139443233612No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG2660.10796024140884057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAC150.002223770169.9924917
AACGGTT207.920485E-569.9924940
GTGAACG150.002223770169.9924912
GGTCATA252.3886046E-455.993991
TACGAGG200.006948535352.4943664
TGCTCGT200.006948535352.49436620
TACGGAC352.08398E-549.99463738
TTGCCTT305.8734673E-446.6616645
GCCGTAT503.4625991E-642.0125554
CGGTGGT602.590914E-740.82895346
CATTAGA350.001254511339.995712
GATACCT459.1756825E-538.8926128
ACTTATG459.186665E-538.8847248
TCGCCGT556.6641223E-638.19322652
GTCGCCG708.6703585E-734.99624651
GTTCGTT708.6703585E-734.99624611
CTCGGTG708.6703585E-734.99624644
TGTTCGT501.7076694E-434.99624610
TTGTTCG501.7076694E-434.9962469
AAGATAC501.7076694E-434.99624626
Produced by FastQC (version 0.11.5)