FastQCFastQC Report
Wed 25 May 2016
SRR1780926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246387
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT235949.575992239850317No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG29821.2102911273727917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC17520.7110764772492055No Hit
CATATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT13260.5381777447673781No Hit
CTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT6210.25204251847703No Hit
ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT5450.22119673521736133TruSeq Adapter, Index 8 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4790.19440960764975423No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC4270.17330459805103354No Hit
CTAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT2740.1112071659624899No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT2720.11039543482407757No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGACGT2720.11039543482407757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG301.0211443E-769.9979729
TTACGAA353.6980055E-969.9979731
CGCCGTA150.002223079669.9979748
TAAGACG207.9174155E-569.9979743
GTAGTCG150.002223079669.9979752
TGCCGTA401.3460522E-1069.9979745
AAGCCGT600.064.1648144
CGTATGA1150.063.91119441
CGAAACT501.4551915E-1162.9981734
AGGGGGG31950.062.80637470
ATCTAGT401.0628355E-861.24822237
GCCGTAT401.0628355E-861.24822246
ATATCGT750.060.66490637
GCCGACT352.966899E-759.9982646
AGCCGTC650.059.22905345
ATCGTAT950.058.94565639
AAACTCG601.8189894E-1258.33164236
ACTCTCG308.36208E-658.33164236
CGACTTC308.36208E-658.33164248
ACGAATC601.8189894E-1258.33164233
Produced by FastQC (version 0.11.5)