FastQCFastQC Report
Wed 25 May 2016
SRR1780925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150607
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4214427.98276308538116No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG2660.17661861666456408No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG150.00221775670.0269154
ACGATTC150.002220680770.00365470
TCTATCG150.002220680770.00365431
TGCGCGT150.002223608569.980415
TCGCCTA200.006938933452.50274335
ATGCTCG200.006938933452.50274334
CACGATT200.006938933452.50274369
TTCCCCG200.006938933452.50274370
GGGAATA200.006938933452.50274338
GAAACTT200.006938933452.50274315
TGCTCGT200.006938933452.50274335
ACCACGT200.006938933452.50274310
TTTGAAC200.006938933452.50274330
TGGGACA200.006938933452.50274310
ACTCAGT200.006938933452.50274345
CCACGTT200.006938933452.50274311
CACCCGC200.006938933452.50274332
GTACATG200.006948055752.485311
TGTGCGC200.006948055752.485313
GACCTAG200.006948055752.4853117
Produced by FastQC (version 0.11.5)