##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 150607 Sequences flagged as poor quality 0 Sequence length 76 %GC 63 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.462056876506406 32.0 27.0 32.0 22.0 32.0 2 29.26869933004442 32.0 32.0 32.0 22.0 32.0 3 29.128506643117518 32.0 32.0 32.0 22.0 32.0 4 28.608683527326086 32.0 27.0 32.0 13.0 32.0 5 28.951250605881533 32.0 32.0 32.0 13.0 32.0 6 32.9947479200834 37.0 32.0 37.0 13.0 37.0 7 32.67111754433725 37.0 32.0 37.0 13.0 37.0 8 32.8623636351564 37.0 32.0 37.0 13.0 37.0 9 33.00598245765469 37.0 32.0 37.0 13.0 37.0 10-11 32.72905641836037 37.0 32.0 37.0 13.0 37.0 12-13 32.57608544091576 37.0 32.0 37.0 13.0 37.0 14-15 33.31031758152011 37.0 34.5 37.0 17.5 37.0 16-17 33.26434030290757 37.0 34.5 37.0 17.5 37.0 18-19 32.71360228940222 37.0 32.0 37.0 13.0 37.0 20-21 33.155155470861246 37.0 34.5 37.0 17.5 37.0 22-23 33.08342573718353 37.0 34.5 37.0 17.5 37.0 24-25 32.74990538288393 37.0 32.0 37.0 13.0 37.0 26-27 33.175061584122915 37.0 34.5 37.0 17.5 37.0 28-29 33.002353808255926 37.0 32.0 37.0 13.0 37.0 30-31 32.84537239304946 37.0 32.0 37.0 13.0 37.0 32-33 32.9479506264649 37.0 32.0 37.0 13.0 37.0 34-35 32.830588883650826 37.0 32.0 37.0 13.0 37.0 36-37 33.36670938269802 37.0 34.5 37.0 17.5 37.0 38-39 33.42401083614971 37.0 34.5 37.0 17.5 37.0 40-41 32.95533740131601 37.0 34.5 37.0 17.5 37.0 42-43 32.81471976734149 37.0 32.0 37.0 13.0 37.0 44-45 32.53541668049958 37.0 32.0 37.0 13.0 37.0 46-47 32.79334293890722 37.0 34.5 37.0 17.5 37.0 48-49 33.2403009156281 37.0 34.5 37.0 17.5 37.0 50-51 33.48756697895848 37.0 37.0 37.0 22.0 37.0 52-53 32.58440842723114 37.0 32.0 37.0 13.0 37.0 54-55 32.72195183490807 37.0 32.0 37.0 13.0 37.0 56-57 32.49797154182741 37.0 32.0 37.0 13.0 37.0 58-59 32.20219179719402 37.0 32.0 37.0 13.0 37.0 60-61 31.93788137337574 37.0 32.0 37.0 13.0 37.0 62-63 31.744719700943513 37.0 32.0 37.0 13.0 37.0 64-65 31.689556262325123 37.0 32.0 37.0 13.0 37.0 66-67 31.77385181299674 37.0 32.0 37.0 13.0 37.0 68-69 31.885782201358502 37.0 32.0 37.0 13.0 37.0 70-71 31.949686933542267 37.0 32.0 37.0 13.0 37.0 72-73 32.29034175038345 37.0 32.0 37.0 13.0 37.0 74-75 32.60056305483809 37.0 32.0 37.0 13.0 37.0 76 32.536017582184094 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.0424440807934765 11101 2 -2.2149754866534117 11101 3 0.3560637433244658 11101 4 0.5417877528338764 11101 5 0.37792109978004973 11101 6 -0.8294261675942991 11101 7 0.5451287484791862 11101 8 -0.32698418760377734 11101 9 0.2509812292246778 11101 10-11 -0.6073754812465921 11101 12-13 0.5556641978076868 11101 14-15 0.4389502039157094 11101 16-17 0.3184179981377824 11101 18-19 0.19520798881170975 11101 20-21 0.04258704140485747 11101 22-23 -0.4755517030194554 11101 24-25 0.2434870352335281 11101 26-27 -0.15022646889085678 11101 28-29 0.5238636864312269 11101 30-31 0.03943448123575877 11101 32-33 0.33091868690252824 11101 34-35 1.6685047393486414 11101 36-37 0.13943495718771715 11101 38-39 -0.5033650038332809 11101 40-41 0.3329493784364672 11101 42-43 -0.02642047565140615 11101 44-45 0.754226718604329 11101 46-47 0.9247148473255749 11101 48-49 0.4407670275577331 11101 50-51 0.8565128191233313 11101 52-53 -0.1247241478966572 11101 54-55 0.11209836987751487 11101 56-57 -0.6354230646442929 11101 58-59 0.2605462298885399 11101 60-61 0.1310938374059667 11101 62-63 0.3767433138290599 11101 64-65 -0.16884457570681022 11101 66-67 0.004225000473493168 11101 68-69 0.07966016704385481 11101 70-71 -0.08539901757193391 11101 72-73 1.3040859367099742 11101 74-75 -1.4454367402234496 11101 76 0.5827067256403495 11102 1 1.0438186389105013 11102 2 0.8391410720884949 11102 3 1.116702041196806 11102 4 0.5239801672649591 11102 5 1.1353216548216807 11102 6 1.1412028795842417 11102 7 0.5171453997280295 11102 8 0.6186679863092692 11102 9 0.8427943652098833 11102 10-11 1.1499094401225136 11102 12-13 1.1403080460963082 11102 14-15 1.0260223593273707 11102 16-17 0.6958463052608153 11102 18-19 1.198676998987466 11102 20-21 0.41076465472585255 11102 22-23 0.9990088890249424 11102 24-25 0.798875564373219 11102 26-27 0.2158697383246846 11102 28-29 1.6762688668197612 11102 30-31 1.0037036764254026 11102 32-33 1.2929908423141896 11102 34-35 0.5662845728361603 11102 36-37 0.19378278327467058 11102 38-39 1.0264684836782791 11102 40-41 0.5451371675206502 11102 42-43 0.8919421515456349 11102 44-45 0.83702042813254 11102 46-47 0.8372957908963414 11102 48-49 -0.33860392526372607 11102 50-51 0.23324732421121297 11102 52-53 1.134757813250431 11102 54-55 1.2500493412003664 11102 56-57 0.18615417864895534 11102 58-59 0.08732698844542597 11102 60-61 1.0455249197371437 11102 62-63 0.7053742111463563 11102 64-65 1.118505100518906 11102 66-67 1.109336008799115 11102 68-69 0.4636795935008422 11102 70-71 -0.6964073431963591 11102 72-73 -0.5458215563520064 11102 74-75 -0.1695347975777466 11102 76 1.3021794360936383 11103 1 1.2066425299952606 11103 2 0.10879412435023994 11103 3 0.745489919984685 11103 4 0.5326538607212612 11103 5 -0.5685650381954481 11103 6 -0.8975542569989798 11103 7 0.8273122859818756 11103 8 -0.8269841876037773 11103 9 -0.9926250462964887 11103 10-11 0.6155989438554883 11103 12-13 0.18982495430886814 11103 14-15 0.7038330751815636 11103 16-17 1.0349986987610365 11103 18-19 0.3815931166861972 11103 20-21 0.1291441013661725 11103 22-23 -0.3937227754458448 11103 24-25 -0.501355002161695 11103 26-27 -0.23055099070474228 11103 28-29 -0.06758269224919644 11103 30-31 0.8598191792800307 11103 32-33 -0.2829863206194929 11103 34-35 -0.06118578289507326 11103 36-37 0.4172127349654957 11103 38-39 -0.05752361118338456 11103 40-41 1.2440282522812964 11103 42-43 0.6329390773251689 11103 44-45 -0.367415232824861 11103 46-47 1.0444762915550996 11103 48-49 0.6100126755267894 11103 50-51 1.481271039626229 11103 52-53 0.43910563933738445 11103 54-55 0.5794850021577673 11103 56-57 -0.3926227207801176 11103 58-59 0.2993384069785847 11103 60-61 0.14302162592950296 11103 62-63 -0.15441937690809837 11103 64-65 0.5347874896273801 11103 66-67 -0.8157298673537134 11103 68-69 0.9778978631538919 11103 70-71 0.19270113286863833 11103 72-73 0.1195275872580055 11103 74-75 0.7822658172663424 11103 76 1.0930226508498961 11104 1 -0.34198986133375797 11104 2 0.5597077550391525 11104 3 0.42849979400579485 11104 4 0.8553187114759986 11104 5 0.6442371408988663 11104 6 0.4261320442154073 11104 7 -0.20696305166424622 11104 8 -1.2876583449071468 11104 9 -1.2196140373304232 11104 10-11 0.7176304953252526 11104 12-13 -0.7396382167273359 11104 14-15 0.2004849875638115 11104 16-17 -0.07418300592628668 11104 18-19 -0.7031305242172223 11104 20-21 -0.3907741825970561 11104 22-23 0.6862340966458547 11104 24-25 -0.3942729456414398 11104 26-27 0.3211457520032326 11104 28-29 -0.6609319148119042 11104 30-31 0.6268717272314603 11104 32-33 0.5699815604382721 11104 34-35 -0.2670080505150949 11104 36-37 0.47651360887310545 11104 38-39 0.8939574919831159 11104 40-41 -0.25353161606508223 11104 42-43 -0.29357323682759073 11104 44-45 0.0075138331154391835 11104 46-47 -0.056817545693789384 11104 48-49 0.8367746775696858 11104 50-51 0.8469503041818953 11104 52-53 0.27618429102277275 11104 54-55 0.22140986880173585 11104 56-57 0.8473046427427491 11104 58-59 0.48705830256364635 11104 60-61 1.0897359602842833 11104 62-63 -0.32454762568803375 11104 64-65 -0.35603080568529677 11104 66-67 -0.06121846115691554 11104 68-69 0.23218228035965183 11104 70-71 1.3131187926121406 11104 72-73 0.3015160108194621 11104 74-75 0.09341097672611198 11104 76 1.408310359396026 11105 1 0.388460711813444 11105 2 0.038087053643170776 11105 3 -0.8302676557728894 11105 4 0.3003306283980258 11105 5 -0.5988680684984793 11105 6 0.3044659450212208 11105 7 0.23135269002227687 11105 8 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11105 70-71 -0.6305311903636834 11105 72-73 0.2659922337226561 11105 74-75 0.5549930899936157 11105 76 -1.1696036117763704 11106 1 -0.249659621959065 11106 2 0.6000106373058856 11106 3 0.5406150846750677 11106 4 -1.4108024414306932 11106 5 0.7548868722129818 11106 6 0.28250722741032774 11106 7 0.49540626929324816 11106 8 0.15127668196144128 11106 9 0.6254030608620553 11106 10-11 0.06838770099207636 11106 12-13 0.2653080460963082 11106 14-15 0.3249354028056288 11106 16-17 -0.01611021647831734 11106 18-19 -0.7143664792734015 11106 20-21 0.7205472634215084 11106 22-23 0.662052367285817 11106 24-25 1.1847451295906097 11106 26-27 1.1560871296290287 11106 28-29 0.5403993016023705 11106 30-31 1.1558775894688793 11106 32-33 0.5212517118794082 11106 34-35 1.2238932684883324 11106 36-37 0.9546523484920613 11106 38-39 -0.6039662989304162 11106 40-41 0.40926760230325954 11106 42-43 0.8919421515456349 11106 44-45 0.83702042813254 11106 46-47 0.4188175300267787 11106 48-49 0.28639607473627393 11106 50-51 0.2006386285590409 11106 52-53 0.19997520455477513 11106 54-55 0.7228754281568897 11106 56-57 1.2350672221272134 11106 58-59 0.728631336271512 11106 60-61 0.8226988327806275 11106 62-63 0.7651568198420193 11106 64-65 1.35220075269282 11106 66-67 0.26694470445128715 11106 68-69 0.05607089784866304 11106 70-71 1.4231578741949455 11106 72-73 0.7748306175610367 11106 74-75 1.4445956372048627 11106 76 0.8347881317458103 11107 1 -1.2467929234401929 11107 2 -1.2702712547151371 11107 3 -0.6497814752867122 11107 4 -1.7159863879189885 11107 5 -0.6877791574095689 11107 6 -2.011329370774096 11107 7 -2.250054791385203 11107 8 -1.3764347370543284 11107 9 -0.9614340151054535 11107 10-11 -2.478196149939599 11107 12-13 -2.0659748014908885 11107 14-15 -0.5910942197457274 11107 16-17 -1.2713061075437722 11107 18-19 -0.12095988586680306 11107 20-21 -1.7095172372951666 11107 22-23 -0.4619323436554126 11107 24-25 0.13517513436843842 11107 26-27 -0.8615311216848696 11107 28-29 -1.6406207652872666 11107 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1 1.4258344491871817 21611 2 0.732026447582566 21611 3 0.7212474957422614 21611 4 0.3973003253677234 21611 5 0.7011319315015214 21611 6 1.2580012985565716 21611 7 1.0141809728505606 21611 8 1.5184703578507666 21611 9 1.363940610269168 21611 10-11 1.743881772138323 21611 12-13 1.5529562674401802 21611 14-15 1.3699946913431802 21611 16-17 1.0294431432054836 21611 18-19 0.3719971570902345 21611 20-21 1.1700531922752617 21611 22-23 1.1219337902107185 21611 24-25 0.9819783311716321 21611 26-27 0.2820752719215207 21611 28-29 1.3541344794679802 21611 30-31 1.328506047966897 21611 32-33 1.002872265239084 21611 34-35 1.0706323989231166 21611 36-37 0.4394349571877143 21611 38-39 1.2606582069984427 21611 40-41 1.5177656260186723 21611 42-43 0.560716855102946 21611 44-45 0.5729888075791791 21611 46-47 0.5596278067066223 21611 48-49 1.4600126755267908 21611 50-51 0.2994528578080491 21611 52-53 0.40728745751920314 21611 54-55 0.40726277993554305 21611 56-57 0.9629328347754367 21611 58-59 1.3513586089987868 21611 60-61 1.8536276865355639 21611 62-63 0.8733584008696766 21611 64-65 1.4085248633647574 21611 66-67 1.4373004356765868 21611 68-69 1.4602210954771238 21611 70-71 1.2664385066060184 21611 72-73 1.10437607210649 21611 74-75 0.09489207989260962 21611 76 1.68696204478929 21612 1 -0.020144571791838928 21612 2 -0.3101064195503014 21612 3 0.15516357965834615 21612 4 -1.3121402340728352 21612 5 -0.1259659705963827 21612 6 -0.8341315685762964 21612 7 -0.08109874742915935 21612 8 -0.23083034144993064 21612 9 -0.46280764147908116 21612 10-11 -0.5770972488407011 21612 12-13 -0.9773759003919906 21612 14-15 -0.7827150988666105 21612 16-17 0.34613894740128615 21612 18-19 -1.0412895561964817 21612 20-21 -0.2926216328995608 21612 22-23 -0.12745432167739779 21612 24-25 -0.3813083821150798 21612 26-27 -1.4521904623442161 21612 28-29 -0.22715922682572653 21612 30-31 0.13497457943543623 21612 32-33 -0.0013235389567185507 21612 34-35 -0.20629067799996648 21612 36-37 0.31731957257233034 21612 38-39 -0.08724389090366458 21612 40-41 -0.13441968866663956 21612 42-43 0.42601405790015434 21612 44-45 0.2918699264602935 21612 46-47 -0.3232393261605182 21612 48-49 -0.7850922195781109 21612 50-51 0.4017255850807757 21612 52-53 -0.49839436066261555 21612 54-55 -0.5173001571273943 21612 56-57 0.3412545130971196 21612 58-59 -0.41079174065156465 21612 60-61 -0.8173513344434582 21612 62-63 -0.6777779627666831 21612 64-65 -0.2282758359359427 21612 66-67 -0.27799676712061583 21612 68-69 0.2753434730994968 21612 70-71 0.6315608842283886 21612 72-73 -0.17228476705435014 21612 74-75 -0.33884917884865473 21612 76 0.8619620447892871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 14.0 12 36.0 13 95.0 14 166.0 15 286.0 16 467.0 17 604.0 18 776.0 19 943.0 20 1159.0 21 1447.0 22 1842.0 23 2342.0 24 2805.0 25 3211.0 26 3636.0 27 4075.0 28 4718.0 29 5330.0 30 6373.0 31 7843.0 32 10048.0 33 14004.0 34 20752.0 35 29768.0 36 27867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 63.17470435515893 13.335059726565873 6.371055025464301 17.1191808928109 2 47.143037377902616 16.59063593564281 20.866949540827505 15.399377145627072 3 45.287078480071195 16.692786846054748 20.82929355982628 17.190841114047785 4 41.87017781496089 14.283627478320918 24.49703199075734 19.34916271596085 5 41.12534278865627 23.61971541071559 24.771086897339362 10.48385490328878 6 50.704159888449915 26.04495202682514 13.822250257295574 9.42863782742937 7 50.46380834124607 21.62070636981162 14.730977881952976 13.18450740698933 8 49.08997948220795 24.23821886972862 14.039747412035778 12.632054236027649 9 47.878860314867566 14.271296056519459 13.788569948805801 24.061273679807176 10-11 49.190581798382496 18.1255893016029 18.385546008685143 14.298282891329464 12-13 49.139960226687954 17.309358680243157 17.500921293329835 16.04975979973905 14-15 48.47328877866423 17.394913065274913 15.976932500672303 18.15486565538856 16-17 48.204823437230246 19.289432794592226 16.28441280760701 16.221330960570526 18-19 46.993143976493634 19.719450854097843 17.82101296502266 15.466392204385862 20-21 47.76263105822671 19.313616775453017 17.320270117728302 15.603482048591975 22-23 48.30130046048984 19.25491615233681 17.222054375649485 15.221729011523866 24-25 47.890424239204776 19.348402047822365 17.121627633649624 15.639546079323235 26-27 48.09716841130245 19.295904172850083 17.54761643908383 15.059310976763642 28-29 47.871266749000704 19.276124457192942 18.16727089226193 14.685337901544425 30-31 48.460562922042136 18.833453956323414 17.563592661695672 15.142390459938781 32-33 47.3735931741974 19.528236114338835 18.081404999834003 15.016765711629759 34-35 47.9852324527475 19.00325696129827 17.881414736338858 15.130095849615374 36-37 48.47526054203367 18.378215067115097 18.257032725872264 14.889491664978967 38-39 47.7671501658328 18.686816707447036 18.684492724272676 14.861540402447487 40-41 48.1190301493023 18.207442870375594 18.465410141400593 15.208116838921518 42-43 47.50556918572031 18.054453522613716 19.123471586363046 15.316505705302927 44-45 47.05251555413903 18.06936116810422 19.152008924125017 15.726114353631734 46-47 47.03550488383057 17.91379756837695 19.15251761299876 15.898179934793724 48-49 46.8273572377158 17.933598937583 19.93227091633466 15.306772908366534 50-51 46.81243816442122 18.01946866222668 19.49057443177668 15.677518741575422 52-53 45.875580167725744 18.791126338784753 19.570991281714175 15.762302211775332 54-55 46.00278875203346 18.790212808339696 18.997045250821685 16.20995318880515 56-57 46.053758657096 18.863339065996453 18.59341695495986 16.48948532194769 58-59 45.94694202802311 18.74128428182482 19.04309715120526 16.268676538946806 60-61 45.757084805184455 18.847440971023346 18.92048019972909 16.474994024063104 62-63 46.126187741117256 18.49157708116148 18.772451710148008 16.609783467573255 64-65 46.5038296685602 18.59555579164744 18.14502607893068 16.755588460861684 66-67 46.57133560870662 18.687167160254454 17.80734139895616 16.934155832082766 68-69 46.71009382657025 18.482107877314956 17.622861013167594 17.184937282947203 70-71 47.62035991765721 18.590211833455076 17.20665382827545 16.58277442061226 72-73 48.11871063701225 18.13295352303928 16.60076426861618 17.14757157133229 74-75 48.37175022326394 18.219333162911294 16.85551420423421 16.553402409590557 76 49.15707769227194 17.27940932360382 16.890317183132257 16.673195800991987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.5 7 2.5 8 3.0 9 2.0 10 4.0 11 9.0 12 11.0 13 11.5 14 19.5 15 16.5 16 26.0 17 46.0 18 71.5 19 101.0 20 111.5 21 118.0 22 143.0 23 212.0 24 286.5 25 317.0 26 428.5 27 612.5 28 800.0 29 915.0 30 1032.0 31 1344.0 32 1743.5 33 1948.0 34 2083.0 35 2532.0 36 3086.5 37 3327.0 38 3681.5 39 4490.0 40 5182.0 41 5729.5 42 6039.0 43 6111.5 44 6496.5 45 6816.5 46 6824.0 47 6664.0 48 6342.5 49 5777.0 50 5373.0 51 4967.5 52 4219.0 53 3613.5 54 3351.0 55 3201.5 56 2696.0 57 2026.0 58 1712.0 59 1521.5 60 1209.0 61 948.5 62 810.0 63 766.0 64 643.0 65 503.0 66 421.5 67 401.0 68 382.5 69 357.5 70 311.5 71 272.0 72 261.5 73 237.0 74 214.0 75 205.0 76 180.0 77 149.5 78 153.5 79 163.0 80 146.0 81 137.5 82 142.5 83 139.0 84 150.5 85 151.5 86 167.5 87 194.0 88 181.5 89 181.5 90 230.0 91 281.0 92 296.0 93 338.5 94 437.5 95 595.0 96 696.0 97 874.5 98 1587.5 99 22555.5 100 42989.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0026559190475874293 2 0.006639797618968574 3 0.013943574999834006 4 6.639797618968573E-4 5 0.0026559190475874293 6 0.0013279595237937146 7 0.003983878571381144 8 0.003983878571381144 9 0.0026559190475874293 10-11 0.003319898809484287 12-13 0.002323929166639001 14-15 0.003651888690432716 16-17 0.005975817857071717 18-19 0.006307807738020145 20-21 0.003983878571381144 22-23 0.004315868452329573 24-25 0.005311838095174859 26-27 0.0016599494047421434 28-29 6.639797618968573E-4 30-31 0.0 32-33 0.0013279595237937146 34-35 0.004315868452329573 36-37 0.00497984821422643 38-39 0.002323929166639001 40-41 0.004315868452329573 42-43 9.95969642845286E-4 44-45 0.0026559190475874293 46-47 0.005311838095174859 48-49 0.004647858333278002 50-51 0.003983878571381144 52-53 0.0026559190475874293 54-55 0.0013279595237937146 56-57 0.005311838095174859 58-59 0.011287655952246576 60-61 0.001991939285690572 62-63 0.003983878571381144 64-65 0.004315868452329573 66-67 0.005975817857071717 68-69 0.006639797618968574 70-71 0.011287655952246576 72-73 0.003651888690432716 74-75 3.3198988094842866E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 150607.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.73254895190795 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31417112299465 29.924239909167564 2 7.9194518716577535 6.293200183258414 3 5.489639037433155 6.54352055349353 4 3.8034759358288768 6.04487175230899 5 2.4649064171122994 4.896850743989323 6 1.5992647058823528 3.812571792811755 7 1.0210561497326203 2.839841441632859 8 0.5882352941176471 1.8697670095015506 9 0.37767379679144386 1.3505348356982079 >10 1.402072192513369 7.825665473716362 >50 0.01671122994652406 0.4395546023757196 >100 0.0016711229946524064 0.17661861666456408 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0016711229946524064 27.98276308538116 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 42144 27.98276308538116 No Hit CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG 266 0.17661861666456408 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016599494047421434 0.0 2 0.0 0.0 0.0 0.019255413095008864 0.0 3 0.0 0.0 0.0 0.019255413095008864 0.0 4 0.0 0.0 0.0 0.01991939285690572 0.0 5 0.0 0.0 0.0 0.01991939285690572 0.0 6 0.0 0.0 0.0 0.01991939285690572 0.0 7 0.0 0.0 0.0 0.021247352380699438 0.0 8 0.0 0.0 0.0 0.021247352380699438 0.0 9 0.0 0.0 0.0 0.021911332142596295 0.0 10 0.0 0.0 0.0 0.03452694761863658 0.0 11 0.0 0.0 0.0 0.03452694761863658 0.0 12 0.0 0.0 0.0 0.036518886904327155 0.0 13 0.0 0.0 0.0 0.03784684642812087 0.0 14 0.0 0.0 0.0 0.041830724999502016 0.0 15 0.0 0.0 0.0 0.04315868452329573 0.0 16 0.0 0.0 0.0 0.04315868452329573 0.0 17 0.0 0.0 0.0 0.04315868452329573 0.0 18 0.0 0.0 0.0 0.04382266428519259 0.0 19 0.0 0.0 0.0 0.0451506238089863 0.0 20 0.0 0.0 0.0 0.05378236071364545 0.0 21 0.0 0.0 0.0 0.05843021904692345 0.0 22 0.0 0.0 0.0 0.06905389523727316 0.0 23 0.0 0.0 0.0 0.07237379404675745 0.0 24 0.0 0.0 0.0 0.08034155118951974 0.0 25 0.0 0.0 0.0 0.08366144999900403 0.0 26 0.0 0.0 0.0 0.09627706547504432 0.0 27 0.0 0.0 0.0 0.1168604380938469 0.0 28 0.0 0.0 0.0 0.13478789166506205 0.0 29 0.0 0.0 0.0 0.1806024952359452 0.0 30 0.0 0.0 0.0 0.21048158452130378 0.0 31 0.0 0.0 0.0 0.2715677226158147 0.0 32 0.0 0.0 0.0 0.3406216178530878 0.0 33 0.0 0.0 0.0 0.405027654757083 0.0 34 0.0 0.0 0.0 0.47872940832763416 0.0 35 0.0 0.0 0.0 0.6135172999926962 0.0 36 0.0 0.0 0.0 0.7815041797526011 0.0 37 0.0 0.0 0.0 1.1134940607010297 0.0 38 0.0 0.0 0.0 1.4786829297443014 0.0 39 0.0 0.0 0.0 1.9394848845007204 0.0 40 0.0 0.0 0.0 2.443445523780435 0.0 41 0.0 0.0 0.0 3.0470031273446785 0.0 42 0.0 0.0 0.0 3.7601173916219035 0.0 43 0.0 0.0 0.0 4.444016546375667 0.0 44 0.0 0.0 0.0 5.147171114224438 0.0 45 0.0 0.0 0.0 5.880204771358569 0.0 46 0.0 0.0 0.0 6.505009727303512 0.0 47 0.0 0.0 0.0 7.187580922533481 0.0 48 0.0 0.0 0.0 7.831641291573433 0.0 49 0.0 0.0 0.0 8.39204021061438 0.0 50 0.0 0.0 0.0 8.995597814178623 0.0 51 0.0 0.0 0.0 9.559980611790953 0.0 52 0.0 0.0 0.0 10.178809749878823 0.0 53 0.0 0.0 0.0 10.814238382014116 0.0 54 0.0 0.0 0.0 11.359365766531436 0.0 55 0.0 0.0 0.0 11.894533454620303 0.0 56 0.0 0.0 0.0 12.371934903424144 0.0 57 0.0 0.0 0.0 12.832072878418666 0.0 58 0.0 0.0 0.0 13.29154687365129 0.0 59 0.0 0.0 0.0 13.781563937931171 0.0 60 0.0 0.0 0.0 14.228422317687757 0.0 61 0.0 0.0 0.0 14.697192029586938 0.0 62 0.0 0.0 0.0 15.217088183152178 0.0 63 0.0 0.0 0.0 15.63672339267099 0.0 64 0.0 0.0 0.0 16.112132902189142 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGACG 15 0.002217756 70.02691 54 ACGATTC 15 0.0022206807 70.003654 70 TCTATCG 15 0.0022206807 70.003654 31 TGCGCGT 15 0.0022236085 69.98041 5 TCGCCTA 20 0.0069389334 52.502743 35 ATGCTCG 20 0.0069389334 52.502743 34 CACGATT 20 0.0069389334 52.502743 69 TTCCCCG 20 0.0069389334 52.502743 70 GGGAATA 20 0.0069389334 52.502743 38 GAAACTT 20 0.0069389334 52.502743 15 TGCTCGT 20 0.0069389334 52.502743 35 ACCACGT 20 0.0069389334 52.502743 10 TTTGAAC 20 0.0069389334 52.502743 30 TGGGACA 20 0.0069389334 52.502743 10 ACTCAGT 20 0.0069389334 52.502743 45 CCACGTT 20 0.0069389334 52.502743 11 CACCCGC 20 0.0069389334 52.502743 32 GTACATG 20 0.0069480557 52.48531 1 TGTGCGC 20 0.0069480557 52.48531 3 GACCTAG 20 0.0069480557 52.48531 17 >>END_MODULE