FastQCFastQC Report
Wed 25 May 2016
SRR1780925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150607
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT2376215.777487102193124No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG31112.065641039261123No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC20071.3326073821269928No Hit
CATATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT12720.8445822571328025No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT12550.8332946011805561No Hit
CTTATACACATCTCCGCGCCCACGAGACCTGACCGAATCTCGTATGCCGT6200.41166745237605157No Hit
ATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTCTT5600.3718286666622401RNA PCR Primer, Index 40 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5190.34460549642446897No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC3970.26359996547305237No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACAGAATCTCGTATGCCGT2910.1932181107119855No Hit
CTAATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT2840.18857025237870748No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGACGT2580.1713067785693892No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT2530.16798687975990492No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTAAGCCGT2020.1341239119031652No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGCATCTCGTATGCCGT1650.10955666071298147No Hit
CTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTC1650.10955666071298147No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATATCGTATGCCGT1640.10889268095108462No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCG1520.10092492380832233No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA150.00222398569.97742548
ACTCCGA500.069.97742511
AGGGGGG30700.064.8918770
TGCCGTA1100.063.6369745
GACAGAA1050.063.35497731
ATCGTAT1250.061.6210539
CCGTAAT401.0586518E-861.25058447
GCCGTAT800.061.25058446
TCAGCAT1050.060.0005753
CGTAATC352.9651164E-759.98064848
CTCGTAA950.058.96751439
AAGCATG308.344448E-658.3338954
TATCGTA1150.057.84584838
CAGCATG1150.057.82663754
GACCGCA850.057.66675631
CGTCTAC553.6379788E-1157.25425348
CAGCTTG2300.056.30488254
CATCTCG1000.056.01913536
TGACCGC750.056.01913530
TTATGCA252.3842338E-456.00053452
Produced by FastQC (version 0.11.5)