FastQCFastQC Report
Wed 25 May 2016
SRR1780924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238157
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3832616.092745541806455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTT150.002222732970.034
CAGTCGT207.915735E-570.01
GCCATAG252.3871759E-455.9999961
CCGGTCG200.00694530452.59
GCGTGTA200.00694530452.516
AGTCGTT200.00694530452.52
CTAGACG200.00695107552.4889825
TGCGGGT200.00695107552.488985
GTCCGTA200.00695107552.4889824
GCCGTAT506.163464E-849.0102954
CCAACGT404.5894725E-543.74081422
TCGCCGT602.5839836E-740.84190852
ATTAGAG350.001253764840.03
GGTCATA350.001253764840.01
GCCCAGT350.001255060439.99160419
CTGCCGG350.001255060439.99160418
TGCCGGC350.001255060439.99160419
CGCGGTA459.1685804E-538.89705743
ACGGCCG459.1685804E-538.89705738
TGGTCGC556.67687E-638.18181649
Produced by FastQC (version 0.11.5)