FastQCFastQC Report
Wed 25 May 2016
SRR1780924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238157
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT225829.48198037429091No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG28571.1996288162850557No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC14400.6046431555654463No Hit
CATATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT14310.6008641358431623No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCCGTGCATCTCGTATGCCGT8000.33591286420302574No Hit
CTTATACACATCTCCGAGCCCACGAGCCCGCCGTGCATCTCGTATGCCGT4580.19231011475623225No Hit
ATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGTCTT4510.18937087719445575RNA PCR Primer, Index 16 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4160.17467468938557337No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC3190.13394525460095652No Hit
CTAATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT3020.1268071062366422No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT2670.11211091842775983No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGACGT2630.11043135410674472No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCGTGCATCTCGTATGCCGT2420.1016136414214153No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC207.9070305E-570.0155551
GCCGACA207.9152625E-570.0008546
TCGAAAG207.9152625E-570.0008540
ACGTGCA500.070.0008431
CGAGCGG150.00222262770.0008437
AGGGGGG32900.063.6311570
AGACCGA501.4551915E-1162.98752625
GACCGAC453.8380676E-1062.2229726
ACCGACG453.8380676E-1062.2229727
CGTCTTA850.061.76544648
ACGCCGT700.060.00071728
TGCCGAC700.060.00071745
AACGCCG601.8189894E-1258.3340327
CGTATGA1400.057.5006941
CCGTCTT38550.056.29120647
GCCGTCT39150.056.23311646
GTCTTCT38300.056.20171749
CGTCTTC38850.056.12680448
CGACGTG509.767973E-1056.00066829
GTTTCCG252.3870343E-456.00066820
Produced by FastQC (version 0.11.5)