FastQCFastQC Report
Wed 25 May 2016
SRR1780923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75093
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4615661.465116588763266No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG3730.49671740375268No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG990.13183652271183732No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG920.12251474837867711No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG830.11052960995032825No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780.10387119971235667No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAT150.00221678870.000016
AATCAGG150.00221678870.0000133
TTATTGG150.00221678870.0000142
TAATGTT150.00221678870.0000144
CTGACTG150.00221678870.000015
CAATCAG150.00221678870.0000132
GTTTCAA150.00221678870.0000148
TAAGAAA150.00221678870.0000152
AACCCAC150.00221678870.0000126
AACCATA150.00221678870.000017
ATAAAGT150.00221678870.0000111
CCACAAT150.00221678870.0000129
GTAATGT150.00221678870.0000143
TAAAGTT150.00221678870.0000112
CTCGTAT150.00221678870.0000160
GTAAAAC150.00221678870.000013
TCGGGCG150.00221678870.0000111
TTGTGCG252.8151862E-670.02
TGTGCGG207.880505E-570.03
GTTGTGC207.880505E-570.01
Produced by FastQC (version 0.11.5)