SRR1780923_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780923_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	75093
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT	28893	38.47628940114258	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG	3952	5.262807452092738	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC	1947	2.592784946666134	No Hit
CATATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT	1544	2.0561170814856244	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	671	0.8935586539357863	No Hit
CTTATACACATCTCCGCGCCCACGAGACGCCAATTCATCTCGTATGCCGT	601	0.8003409106041841	No Hit
ATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCTT	535	0.7124498954629592	RNA PCR Primer, Index 45 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC	417	0.5553114138468299	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGACGT	388	0.5166926344665947	No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT	313	0.41681648089702106	No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT	305	0.40616302451626646	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCAGT	252	0.3355838759937677	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATATCGTATGCCGT	216	0.2876433222803723	No Hit
CTTATACACATCTCCGAGCCCACGAGCCGCCAATTCATCTCGTATGCCGT	213	0.28364827613758936	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAAATCATCTCGTATGCCGT	182	0.24236613266216556	Illumina PCR Primer Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTC	167	0.22239090194825084	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGA	155	0.20641071737711905	No Hit
ACTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG	145	0.19309389690117587	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTAATCTCGTATGCCGT	144	0.19176221485358155	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG	136	0.181108758472827	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACAATTCATCTCGTATGCCGT	129	0.1717869841396668	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTAAGCCGT	122	0.16246520980650658	No Hit
TCTTATACACATCTCCGCGCCCACGAGACGCCAATTCATCTCGTATGCCG	118	0.15713848161612934	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCAAATTCATCTCGTATGCCGT	115	0.15314343547334638	No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC	110	0.14648502523537482	No Hit
CTTTACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTC	108	0.14382166114018616	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGAATGCCGT	107	0.14248997909259187	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCCTCTCGTATGCCGT	106	0.14115829704499755	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATACATCTCGTATGCCGT	105	0.1398266149974032	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG	94	0.12517811247386573	No Hit
CTTATACACATCACCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT	82	0.10919792790273396	No Hit
TATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCT	76	0.10120783561716805	RNA PCR Primer, Index 45 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACAACA	20	7.857095E-5	70.042	7
CACTTAT	15	0.0022115153	70.042	1
AACATTA	15	0.0022115153	70.042	6
CAGCTAG	20	7.883106E-5	69.99534	54
CCCAGGC	20	7.883106E-5	69.99534	48
TACAGCT	30	1.0117583E-7	69.99534	52
GAGGAAC	15	0.0022173738	69.99534	34
CTGGGTG	15	0.0022173738	69.99534	36
CCAAATA	15	0.0022173738	69.99534	30
CCAGTGG	20	7.883106E-5	69.99534	14
CAGTATT	15	0.0022173738	69.99534	47
TAAGCCG	55	0.0	69.99534	43
TAACGTA	15	0.0022173738	69.99534	38
ATGGTTT	15	0.0022173738	69.99534	44
GGTTTTA	15	0.0022173738	69.99534	46
ACACCGA	20	7.883106E-5	69.99534	11
GTTTTAC	15	0.0022173738	69.99534	47
CAACACC	20	7.883106E-5	69.99534	9
TCTACAG	30	1.0117583E-7	69.99534	50
ATAACGT	15	0.0022173738	69.99534	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content