FastQCFastQC Report
Wed 25 May 2016
SRR1780922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305882
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3302310.795993226146031No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACT200.00694404152.5060148
GCGACCA200.006948532552.4974234
TGGGTCG901.8189894E-1242.77567712
CGTGCCC350.001253618340.0045847
GGACCTA350.001253618340.004581
GACTCGC350.001253618340.0045844
TGACTCG350.001253618340.0045843
CCGATAG350.001254626739.99804330
CTACCCG350.001254626739.99804310
CGAATGG459.1887225E-538.88698250
AACAGTG556.6853518E-638.1799477
GCCGTAT753.5292032E-837.331554
TGACCTT501.7064065E-435.0040147
TATACGA501.7064065E-435.0040146
TGGTCGC1003.237801E-1035.0040149
GCTGACC400.00241531135.00400545
GGGACAT400.00241531135.0040051
CGTTCGG708.6755244E-734.99828724
TCGCCGT806.22822E-834.99828352
GTCGCCG904.489266E-934.99828351