FastQCFastQC Report
Wed 25 May 2016
SRR1780922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305882
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT212756.955296486880562No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG29290.9575587971832275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC16720.5466160153261715No Hit
CATATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT12220.39950046096207037No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4790.15659633453423216No Hit
CTTATACACATCTCCGCGCCCACGAGACTAATGTCTATCTCGTATGCCGT4510.14744247781824366No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT4430.14482709018510406RNA PCR Primer, Index 15 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC3790.12390398911998744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGCG150.00222241270.0073612
TACGGAA150.002223853769.9959228
AAGCCGT301.0221811E-769.9959244
AGGGGGG27300.065.5197170
CGTCTAC453.8380676E-1062.2185948
ATATCGT750.060.66312837
ATCGTAT950.058.94392839
ACGTATG308.36835E-658.3299340
GCAGCGT252.3870135E-456.0058861
AGCGTCA252.3889431E-455.996733
GCCGTCT34750.054.08317646
CGTCTTC35000.053.9968548
CGTATGC36150.053.9249341
CCGTCTT34500.053.76497747
TATGCCG35700.053.7223643
CTTGAAA35250.053.72196257
TCGTATG37100.053.67611340
ATGCCGT35450.053.6075944
TCTCGTA36000.053.56631538
TGCCGTC35350.053.5612345