FastQCFastQC Report
Wed 25 May 2016
SRR1780921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174498
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3327419.068413391557495No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG2420.1386835379202054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGCA150.002221986469.997997
CGAGCAT252.3823588E-456.01445435
CGAGACT200.00693512352.51354639
TAATTGC200.00694299252.49849710
TACCGAC200.00694299252.49849770
AATTTAG305.866454E-446.66532556
ACGTAGA305.866454E-446.66532524
TCGCCGT503.46456E-641.998852
GATCTCG602.577708E-740.8438741
CTCGGTG602.5835106E-740.8321644
GAGCATA350.001251254240.01032336
ATCTCGG701.9126674E-839.99885642
ACACACG350.0012530239.99885630
CACGAGC350.0012530239.99885633
GCCGTAT556.6679204E-638.18072554
AGATCTC654.8287984E-737.70203440
GTAGATC654.8287984E-737.70203438
GTCGCCG654.839676E-737.69122751
GGTATAG951.8553692E-1036.8410531
GTTATCT400.002410776435.0090336
Produced by FastQC (version 0.11.5)