FastQCFastQC Report
Wed 25 May 2016
SRR1780921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174498
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT1946311.15370949810313No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG24421.3994429735584362No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC14430.8269435752845306No Hit
CATATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT12260.7025868491329413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4670.2676248438377517No Hit
ATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGTCTT4540.2601749017180713RNA PCR Primer, Index 31 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACCCCGAGATATCTCGTATGCCGT4340.24871345230317826No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGAGATATCTCGTATGCCGT4100.23495971300530666No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC2830.16217950922073607No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT2490.14269504521541795No Hit
CTAATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT2290.13123359580052493No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCCGAGATATCTCGTATGCCGT2280.1306605233297803No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGACGT2080.11919907391488727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTT150.002222094769.997135
CCGATAG150.002222094769.997139
AGTGGAC150.002222094769.9971312
TAACCGC150.002222094769.9971317
GCCGACA150.002222094769.9971346
ACCCGAG252.8315862E-669.9971328
GCCGTAT207.911415E-569.9971346
AGGGGGG25750.064.5789770
CGTCTTA550.063.63375548
GATAACT453.8198777E-1062.21967334
AACTCGT352.962097E-759.9975437
TGACGTC650.059.22834445
CGTATGA1000.056.01376341
AATCTCG509.749783E-1055.99770736
GTCTTCA1450.055.5149749
GCCGTCT32400.053.47003646
CGTCTTC31950.053.4563448
CTCGTAT34300.053.38046339
TCGTATG34700.053.3704640
TATGCCG34050.053.3611343
Produced by FastQC (version 0.11.5)