FastQCFastQC Report
Wed 25 May 2016
SRR1780920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58407
Sequences flagged as poor quality0
Sequence length76
%GC89

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3960867.81378944304622No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG3230.55301590562775No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1340.22942455527590871No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950.16265173694933827No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG840.14381837793415173No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG760.1301213895594706No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.10786378345061379No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGT150.002204640370.0771954
CCGGGTC150.002204640370.0771955
GTCTGGC207.856284E-570.01713610
GCTGCGG150.002212158270.01713617
GCGTCTG150.002212158270.0171368
CGCGTCT150.002212158270.0171367
CGTCTGG207.856284E-570.0171369
CTCGGTG308.26924E-658.34761444
TCTGGCG252.3693434E-456.0137111
TCTCGGT252.3693434E-456.0137143
GATCTCG252.3693434E-456.0137141
AGATCTC252.3693434E-456.0137140
GTCGGGC252.3693434E-456.0137110
TCGGGCG252.3693434E-456.0137111
GTCGCCG452.6395355E-854.4577751
TGCGCGT200.006912482452.512855
CTCTAGG200.006912482452.512852
CATTAAA407.349081E-752.5128561
CTTGTGC200.006912482452.512851
TGGCGCT200.006912482452.5128513