FastQCFastQC Report
Wed 25 May 2016
SRR1780920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58407
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT2497242.75514921156711RNA PCR Primer, Index 14 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG32065.489068091153458No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC18903.2359135035184137RNA PCR Primer, Index 14 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT14392.463745783895766No Hit
CTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT8191.4022291848579793RNA PCR Primer, Index 14 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6191.0598044754909515No Hit
ATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCTT4920.8423647850428886RNA PCR Primer, Index 14 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC3980.6814251716403856RNA PCR Primer, Index 14 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT3090.5290461759720582No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGACGT3080.527334052425223No Hit
CTTAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT3030.5187734346910473RNA PCR Primer, Index 14 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATATCGTATGCCGT2290.392076292225247No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGATGCATCTCGTATGCCGT2060.35269745064803876No Hit
CTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC1720.294485250055644No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCAGT1440.24654579074426008No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTAAGCCGT1440.24654579074426008No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTAGCATCTCGTATGCCGT1390.2379851730100844RNA PCR Primer, Index 24 (95% over 23bp)
TCTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCG1370.23456092591641414No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG1190.20374270207338163No Hit
ACTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG1180.20203057852654646No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGA1170.20031845497971135RNA PCR Primer, Index 14 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTAGTATGCCGT990.1695002311366788No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGAATGCCGT990.1695002311366788No Hit
CATATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT970.16607598404300855No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG950.16265173694933827No Hit
CTTTACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC920.15751536630883287RNA PCR Primer, Index 14 (95% over 24bp)
CATAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT880.1506668721214923No Hit
CTTATACACATCTCCGAGCCAACGAGACACAGTTGCATCTCGTATGCCGT880.1506668721214923No Hit
CTTATACACATCACCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT840.14381837793415173RNA PCR Primer, Index 14 (95% over 23bp)
CACTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC820.14039413084048144RNA PCR Primer, Index 14 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAGTTGAATCTCGTATGCCGT820.14039413084048144TruSeq Adapter, Index 8 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCT780.1335456366531409RNA PCR Primer, Index 14 (96% over 25bp)
CAAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT750.12840926601263547RNA PCR Primer, Index 14 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC720.12327289537213004No Hit
CTTATACACATCTCCGAGACCACGAGACACAGTTGCATCTCGTATGCCGT690.11813652473162463No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC640.10957590699744893No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGTTGCATCTCGTATGCCGT590.10101528926327323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA252.7961178E-670.04713448
CTTCAGA252.7961178E-670.04713451
CGTCAAC207.839638E-570.04713448
CTGATTG401.3096724E-1070.04713454
ATCTGAT252.7961178E-670.04713452
CAAAAAC401.3096724E-1070.0471341
GTCATAA150.002208404270.0471349
GTATTAT150.002208404270.0471349
TCAACTG150.002208404270.0471350
ACATCAG150.002208404270.0471350
ATTATGC150.002208404270.0471351
GACATCA150.002208404270.0471349
AAAAGCG150.002208404270.0471367
CAGATTG150.002208404270.0471354
AATGTGG150.002208404270.0471369
AGTCATC150.002208404270.0471348
AAATGTG150.002208404270.0471368
AGAAGCA207.873021E-569.9871631
CAGTATT207.873021E-569.9871647
ACGCAGT207.873021E-569.9871627
Produced by FastQC (version 0.11.5)