##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780920_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58407 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.900217439690447 32.0 32.0 32.0 27.0 32.0 2 29.502919170647353 32.0 32.0 32.0 22.0 32.0 3 30.368294896159707 32.0 32.0 32.0 27.0 32.0 4 30.751570873354222 32.0 32.0 32.0 27.0 32.0 5 29.82303491019912 32.0 32.0 32.0 27.0 32.0 6 32.08961254644135 37.0 32.0 37.0 13.0 37.0 7 34.67288167514168 37.0 37.0 37.0 32.0 37.0 8 32.908572602599 37.0 32.0 37.0 22.0 37.0 9 34.40916328522266 37.0 37.0 37.0 27.0 37.0 10-11 33.242642149057474 37.0 34.5 37.0 20.0 37.0 12-13 34.50457993048778 37.0 37.0 37.0 27.0 37.0 14-15 34.25988323317411 37.0 37.0 37.0 27.0 37.0 16-17 30.784563494101732 34.5 27.0 37.0 17.5 37.0 18-19 33.18462684267297 37.0 34.5 37.0 17.5 37.0 20-21 34.198417997842725 37.0 37.0 37.0 27.0 37.0 22-23 32.66194976629514 37.0 32.0 37.0 20.0 37.0 24-25 33.65205369219443 37.0 34.5 37.0 24.5 37.0 26-27 33.712192031777015 37.0 34.5 37.0 22.5 37.0 28-29 33.46985806495796 37.0 34.5 37.0 20.0 37.0 30-31 34.433843546150285 37.0 37.0 37.0 29.5 37.0 32-33 34.22421113907579 37.0 37.0 37.0 27.0 37.0 34-35 33.60234218501207 37.0 34.5 37.0 22.0 37.0 36-37 31.1253188830106 34.5 29.5 37.0 17.5 37.0 38-39 28.926883763932405 34.5 22.0 37.0 8.0 37.0 40-41 30.71010324104987 37.0 27.0 37.0 10.5 37.0 42-43 32.645196637389354 37.0 32.0 37.0 13.0 37.0 44-45 32.7381392641293 37.0 32.0 37.0 13.0 37.0 46-47 31.946350608659923 37.0 29.5 37.0 13.0 37.0 48-49 31.308901330319998 37.0 29.5 37.0 13.0 37.0 50-51 31.661102265139455 37.0 29.5 37.0 13.0 37.0 52-53 30.399558272124917 37.0 27.0 37.0 10.5 37.0 54-55 30.038676870923005 37.0 24.5 37.0 13.0 37.0 56-57 31.072936463095175 37.0 27.0 37.0 13.0 37.0 58-59 30.260559521975107 37.0 24.5 37.0 13.0 37.0 60-61 31.923819062783572 37.0 32.0 37.0 13.0 37.0 62-63 33.20012156077182 37.0 32.0 37.0 22.0 37.0 64-65 33.312924820655056 37.0 32.0 37.0 22.0 37.0 66-67 32.82431900285924 37.0 32.0 37.0 17.5 37.0 68-69 31.222182272672796 37.0 29.5 37.0 13.0 37.0 70-71 27.01736093276491 32.0 17.5 37.0 10.5 37.0 72-73 30.77066105090143 37.0 27.0 37.0 13.0 37.0 74-75 32.97148458232746 37.0 34.5 37.0 13.0 37.0 76 33.79903093807249 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.36342595818641854 11101 2 -1.8942020464930351 11101 3 -0.29115018992769137 11101 4 0.24996388980578033 11101 5 0.6603562378420449 11101 6 -1.693039972341257 11101 7 -0.7887347755793996 11101 8 0.1441639726921764 11101 9 -0.10029275953967698 11101 10-11 -2.521444809950925 11101 12-13 -0.16121423146841352 11101 14-15 -1.674458501189548 11101 16-17 -3.591435693044353 11101 18-19 -0.5586392576846819 11101 20-21 -0.5259640754305437 11101 22-23 0.7843969988133992 11101 24-25 0.9928461127677082 11101 26-27 0.08632976657129632 11101 28-29 -2.395957908601666 11101 30-31 -2.714379357766763 11101 32-33 -2.4021909269829678 11101 34-35 0.24015819227425794 11101 36-37 -0.3659540255016722 11101 38-39 -2.1276206099378676 11101 40-41 -2.460482113194125 11101 42-43 -1.8742827205890862 11101 44-45 -0.2686970428011648 11101 46-47 -1.3039072311183624 11101 48-49 -0.45190940457528583 11101 50-51 -0.9964493379811223 11101 52-53 -4.256096117452742 11101 54-55 -2.0693917664938475 11101 56-57 -1.773243447162276 11101 58-59 -0.7277847903956314 11101 60-61 -2.4789078862656417 11101 62-63 -0.04294998783073822 11101 64-65 0.5410088408330367 11101 66-67 -0.47180382533016285 11101 68-69 -1.3052954555095404 11101 70-71 -1.830019825316537 11101 72-73 1.2455082068988865 11101 74-75 -1.4988653245503976 11101 76 1.3937715288424783 11102 1 -0.3413359465754837 11102 2 1.3301365778455896 11102 3 -0.09115018992769208 11102 4 -0.19024775040586306 11102 5 -2.143876566390759 11102 6 -0.19780187710316 11102 7 0.5763445894999677 11102 8 -0.3468413183131105 11102 9 -1.9542610135079315 11102 10-11 -0.4611273496334647 11102 12-13 -0.08184915210332377 11102 14-15 0.06998594325489194 11102 16-17 1.0715272699186116 11102 18-19 1.15987926083384 11102 20-21 -0.31855666802314175 11102 22-23 1.1140266284430353 11102 24-25 -0.6055665856449934 11102 26-27 0.29532447556600516 11102 28-29 0.36700505436129305 11102 30-31 0.16181111842371365 11102 32-33 1.438549813757767 11102 34-35 -0.5762439241278585 11102 36-37 -0.12785878740643852 11102 38-39 -0.20434018665744702 11102 40-41 -0.8530747057867174 11102 42-43 0.45481780851144293 11102 44-45 -1.2718716459757644 11102 46-47 0.8648758376647088 11102 48-49 -0.9053485580144418 11102 50-51 1.604079762547979 11102 52-53 0.4269726656160415 11102 54-55 0.4374865403844552 11102 56-57 1.7669681930493617 11102 58-59 1.3251252625144225 11102 60-61 -0.34769095504871217 11102 62-63 -0.6683468132275721 11102 64-65 -1.620895921071721 11102 66-67 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11104 24-25 -1.312709442787849 11104 26-27 -1.2993183815768532 11104 28-29 -0.17049494563870127 11104 30-31 0.21895397556656349 11104 32-33 0.2385498137577713 11104 34-35 -1.9691010669850044 11104 36-37 0.9132126411649892 11104 38-39 -1.0632687580860178 11104 40-41 -1.8530747057867174 11104 42-43 -0.3094679057742695 11104 44-45 -4.3450859316900505 11104 46-47 -1.97798130519244 11104 48-49 -1.1857057008715834 11104 50-51 -0.3441345231663071 11104 52-53 -0.46767019152681755 11104 54-55 -0.014299173901257234 11104 56-57 -1.797317521236355 11104 58-59 -0.8088033089141504 11104 60-61 -2.8673338121915677 11104 62-63 -1.054061098941851 11104 64-65 -1.092324492500289 11104 66-67 0.4068998783735438 11104 68-69 -0.8006658258799142 11104 70-71 -1.1962235290202408 11104 72-73 -0.929954756064074 11104 74-75 -1.2696986578837297 11104 76 -3.3812284711575202 11105 1 -1.457086862326399 11105 2 -2.9511821034730943 11105 3 -0.7783296771071768 11105 4 -1.1631415232996325 11105 5 -1.473546896061091 11105 6 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66-67 -1.7039975575238913 11105 68-69 0.1320264818123924 11105 70-71 0.9662764709797607 11105 72-73 0.6158785772692568 11105 74-75 0.6165192908342192 11105 76 -0.19597206090111285 11106 1 1.3721978880109802 11106 2 0.5346478560410759 11106 3 1.314112967967045 11106 4 -0.5706988782254108 11106 5 -1.410793859623844 11106 6 -0.44742593725353785 11106 7 0.3808558676954519 11106 8 -0.21451049124544141 11106 9 -0.027193344335003644 11106 10-11 -0.645713815798878 11106 12-13 -0.9521499039830275 11106 14-15 -0.8352772146398451 11106 16-17 -0.04952536166033639 11106 18-19 -0.36906810758721775 11106 20-21 0.11828543724001861 11106 22-23 -1.6780786347148648 11106 24-25 -2.44428839015627 11106 26-27 -0.838134171050541 11106 28-29 -0.40667915616501915 11106 30-31 -0.3415723402229034 11106 32-33 -0.6746080809790698 11106 34-35 -0.29541685645868654 11106 36-37 0.4191336937965744 11106 38-39 0.5926522945455659 11106 40-41 -2.289916811049874 11106 42-43 -1.6515731689321633 11106 44-45 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44-45 0.8424140683099495 11109 46-47 1.4077329805218461 11109 48-49 2.180365727699847 11109 50-51 -0.6816345231663057 11109 52-53 0.14125837990175327 11109 54-55 -0.27679917390125297 11109 56-57 0.8098253359065062 11109 58-59 0.4679824053715649 11109 60-61 -0.39054809790585665 11109 62-63 -0.9540610989418497 11109 64-65 1.3076755074997095 11109 66-67 -1.110957264483595 11109 68-69 -0.40423725445134195 11109 70-71 0.12341932812261902 11109 72-73 1.825402386793069 11109 74-75 0.6820870564019827 11109 76 0.05091438598533671 11110 1 -0.7266641319036715 11110 2 0.7494415971506072 11110 3 -1.0208799196574212 11110 4 0.37809201793390557 11110 5 1.3310269085127153 11110 6 -0.7020489813502664 11110 7 -0.458404445249073 11110 8 -0.49047066194245303 11110 9 -1.157349816596735 11110 10-11 -1.1325559210620355 11110 12-13 -0.3038568741110552 11110 14-15 0.9051210783900316 11110 16-17 -1.9798240814327457 11110 18-19 1.3787981797527564 11110 20-21 0.6246865752201032 11110 22-23 1.5221347365511448 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-0.08510893141529863 21612 44-45 0.6372858631817451 21612 46-47 0.7502238230126927 21612 48-49 1.6364096837438034 21612 50-51 0.5875962460644608 21612 52-53 1.6441887828321597 21612 54-55 2.39499569789362 21612 56-57 2.114220940302115 21612 58-59 -0.4514315140423548 21612 60-61 1.2618328544751023 21612 62-63 -0.2694457143264657 21612 64-65 1.182034481858686 21612 66-67 1.898566545040218 21612 68-69 1.6961290459149616 21612 70-71 2.632943137646425 21612 72-73 1.1671606285513079 21612 74-75 1.2319039062188324 21612 76 -0.811356676285726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 6.0 19 30.0 20 67.0 21 168.0 22 346.0 23 593.0 24 876.0 25 1195.0 26 1589.0 27 1979.0 28 2490.0 29 3173.0 30 3881.0 31 5241.0 32 7146.0 33 9886.0 34 11729.0 35 7293.0 36 719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 7.308970099667775 6.514367914511765 10.941192588279618 75.23546939754084 2 4.446537111548668 12.801986131324373 68.70302200154096 14.048454755585995 3 4.819627784340918 10.817196568904413 74.94307189206774 9.420103754686938 4 3.7563990617562966 68.72977554060301 20.079784957282516 7.434040440358176 5 3.732429332100604 19.757905730477514 72.6128717448251 3.896793192596778 6 6.200130132529708 76.42546488133968 11.905414198143898 5.468990787986713 7 5.994657717201466 16.600458888394236 9.456867915482347 67.94801547892195 8 5.4616741144040954 76.28708887633331 4.958309791634565 13.292927217628023 9 5.326507550594117 13.652706913673251 4.2838064582405915 76.73697907749204 10-11 5.633742530861027 44.298628589038984 37.13082336021367 12.936805519886315 12-13 6.322286810091689 8.253644839010693 43.68670222328759 41.73736612761003 14-15 6.46754669816974 6.048076429195131 11.17417432841954 76.31020254421558 16-17 41.986627971680754 35.93839621268545 4.163135321159822 17.911840494473978 18-19 44.5477425651035 12.946222199393908 4.4292636156625065 38.07677161984009 20-21 9.043044996918018 6.285528388466544 4.474864735292104 80.19656187932334 22-23 6.71751673600767 42.16960295854949 4.270892187580256 46.841988117862584 24-25 42.14941830104526 41.11785503325828 4.195594668401633 12.53713199729482 26-27 44.831689610300664 45.13303883295664 4.350729402095746 5.684542154646942 28-29 8.984675969523158 45.33344747881174 4.575806865850526 41.106069685814575 30-31 5.750166932045817 44.84051569161231 6.030955193726779 43.3783621826151 32-33 42.357674856604746 12.910709699512028 36.59104528721856 8.140570156664669 34-35 40.607290222062424 6.298902528806479 48.1046792336535 4.989128015477597 36-37 12.625307114790305 37.42477292767072 8.758442981517469 41.191476976021505 38-39 6.094355936411187 13.580680232508369 40.70694186434729 39.61802196673315 40-41 5.67422030836665 6.818823891994624 44.15498805742708 43.35196774221164 42-43 41.843880556126294 5.568967878912403 40.528046024244915 12.05910554071639 44-45 8.41459134142061 41.47111951990001 44.041983066373305 6.0723060723060724 46-47 41.49287720019177 8.914629134990754 13.513629203479214 36.07886446133827 48-49 39.7726299929803 5.4573938055370075 6.773160751279812 47.99681545020288 50-51 11.637602623040639 6.383816592615421 41.93355077860818 40.04503000573576 52-53 5.130553890933996 8.244157178323773 74.4739320263676 12.151356904374627 54-55 4.581917179621085 8.609073086373249 45.95443766212641 40.854572071879254 56-57 40.712995385392496 5.793515577510852 13.62978690615823 39.863702130938414 58-59 7.829087048832271 9.93853160742415 69.99948633655228 12.232895007191289 60-61 40.32668156252407 39.49028772975148 14.764020511766871 5.419010195957572 62-63 7.329600904001232 82.69642337391066 4.375331723937199 5.598643998150907 64-65 4.3097839115098795 85.85836101503374 4.094894010479093 5.736961062977295 66-67 4.58693604999572 85.70841537539594 3.9234654567245952 5.781183117883742 68-69 6.405438030255037 84.27919559615434 3.6556028320220535 5.65976354156857 70-71 33.76454371891133 56.59614907151358 3.8689074766915237 5.770399732883573 72-73 81.86028593442343 8.774933652940673 3.78049824501327 5.584282167622636 74-75 85.7145303096455 4.837729323437006 3.9405535437587216 5.507186823158778 76 86.21427715278848 4.698549682368452 3.662608516977449 5.424564647865618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 4.0 17 8.0 18 5.5 19 5.0 20 15.0 21 23.0 22 20.5 23 21.0 24 29.5 25 35.0 26 45.0 27 75.0 28 102.0 29 109.0 30 120.5 31 143.0 32 184.5 33 215.0 34 242.5 35 271.5 36 325.5 37 378.0 38 404.5 39 467.0 40 578.5 41 755.5 42 857.0 43 1011.5 44 1473.0 45 2656.0 46 3532.0 47 7734.5 48 19359.5 49 14895.5 50 3009.0 51 2150.5 52 954.0 53 535.0 54 454.0 55 433.0 56 409.0 57 372.5 58 339.0 59 311.5 60 255.0 61 212.5 62 199.0 63 213.0 64 201.5 65 149.0 66 120.0 67 118.0 68 98.5 69 78.5 70 72.5 71 67.0 72 57.5 73 41.5 74 32.0 75 29.0 76 23.5 77 15.0 78 14.0 79 16.0 80 14.0 81 10.5 82 7.5 83 6.0 84 6.0 85 5.0 86 3.5 87 3.0 88 2.0 89 2.5 90 3.0 91 1.0 92 0.0 93 1.0 94 2.5 95 3.5 96 4.0 97 5.0 98 22.5 99 321.0 100 603.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022257606108856815 2 0.003424247093670279 3 0.0 4 0.0 5 0.0 6 0.008560617734175699 7 0.008560617734175699 8 0.0 9 0.0017121235468351395 10-11 0.0 12-13 0.0042803088670878495 14-15 0.0 16-17 0.0025681853202527095 18-19 0.0 20-21 0.005136370640505419 22-23 0.0 24-25 8.560617734175698E-4 26-27 0.005136370640505419 28-29 0.003424247093670279 30-31 0.0 32-33 0.003424247093670279 34-35 0.0 36-37 8.560617734175698E-4 38-39 8.560617734175698E-4 40-41 0.0042803088670878495 42-43 0.005136370640505419 44-45 0.0042803088670878495 46-47 0.005136370640505419 48-49 0.0 50-51 0.0025681853202527095 52-53 0.003424247093670279 54-55 0.0059924324139229886 56-57 0.009416679507593268 58-59 0.005136370640505419 60-61 0.0025681853202527095 62-63 0.0 64-65 0.008560617734175699 66-67 0.003424247093670279 68-69 0.0059924324139229886 70-71 0.009416679507593268 72-73 0.003424247093670279 74-75 0.0025681853202527095 76 0.010272741281010838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 58407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.478589895046827 #Duplication Level Percentage of deduplicated Percentage of total 1 79.29055400557992 17.030492920369134 2 8.170585890793145 3.509853271012036 3 4.376245516141889 2.8198674816374747 4 2.9095257074531684 2.4997003783793037 5 1.745715424471901 1.874775283784478 6 0.9804703068951772 1.263547177564333 7 0.629732961339179 0.9468043213998322 8 0.40653646871263455 0.6985464071087369 9 0.15942606616181745 0.3081822384303251 >10 1.00438421681945 4.287157361275189 >50 0.15942606616181745 2.6452308798602906 >100 0.11956954962136308 5.7099320286951905 >500 0.015942606616181746 2.4620336603489306 >1k 0.023913909924272617 11.188727378567638 >5k 0.0 0.0 >10k+ 0.007971303308090873 42.75514921156711 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT 24972 42.75514921156711 RNA PCR Primer, Index 14 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG 3206 5.489068091153458 No Hit CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC 1890 3.2359135035184137 RNA PCR Primer, Index 14 (95% over 21bp) CATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT 1439 2.463745783895766 No Hit CTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT 819 1.4022291848579793 RNA PCR Primer, Index 14 (95% over 23bp) GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 619 1.0598044754909515 No Hit ATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCTT 492 0.8423647850428886 RNA PCR Primer, Index 14 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC 398 0.6814251716403856 RNA PCR Primer, Index 14 (95% over 21bp) CTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT 309 0.5290461759720582 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGACGT 308 0.527334052425223 No Hit CTTAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT 303 0.5187734346910473 RNA PCR Primer, Index 14 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATATCGTATGCCGT 229 0.392076292225247 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGATGCATCTCGTATGCCGT 206 0.35269745064803876 No Hit CTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC 172 0.294485250055644 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCAGT 144 0.24654579074426008 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTAAGCCGT 144 0.24654579074426008 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGTAGCATCTCGTATGCCGT 139 0.2379851730100844 RNA PCR Primer, Index 24 (95% over 23bp) TCTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCG 137 0.23456092591641414 No Hit CTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG 119 0.20374270207338163 No Hit ACTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG 118 0.20203057852654646 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGA 117 0.20031845497971135 RNA PCR Primer, Index 14 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTAGTATGCCGT 99 0.1695002311366788 No Hit CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGAATGCCGT 99 0.1695002311366788 No Hit CATATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT 97 0.16607598404300855 No Hit CCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG 95 0.16265173694933827 No Hit CTTTACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC 92 0.15751536630883287 RNA PCR Primer, Index 14 (95% over 24bp) CATAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT 88 0.1506668721214923 No Hit CTTATACACATCTCCGAGCCAACGAGACACAGTTGCATCTCGTATGCCGT 88 0.1506668721214923 No Hit CTTATACACATCACCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT 84 0.14381837793415173 RNA PCR Primer, Index 14 (95% over 23bp) CACTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC 82 0.14039413084048144 RNA PCR Primer, Index 14 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACAGTTGAATCTCGTATGCCGT 82 0.14039413084048144 TruSeq Adapter, Index 8 (95% over 23bp) TATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCT 78 0.1335456366531409 RNA PCR Primer, Index 14 (96% over 25bp) CAAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT 75 0.12840926601263547 RNA PCR Primer, Index 14 (95% over 23bp) TTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTC 72 0.12327289537213004 No Hit CTTATACACATCTCCGAGACCACGAGACACAGTTGCATCTCGTATGCCGT 69 0.11813652473162463 No Hit TCTTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC 64 0.10957590699744893 No Hit CTTATACACATCTCCGAGCCCACGAGACAAAGTTGCATCTCGTATGCCGT 59 0.10101528926327323 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010272741281010838 0.0 2 0.0 0.0 0.0 0.010272741281010838 0.0 3 0.0 0.0 0.0 0.010272741281010838 0.0 4 0.0 0.0 0.0 0.010272741281010838 0.0 5 0.0 0.0 0.0 0.027393976749362232 0.0 6 0.0 0.0 0.0 0.027393976749362232 0.0 7 0.0 0.0 0.0 0.027393976749362232 0.0 8 0.0 0.0 0.0 0.027393976749362232 0.0 9 0.0 0.0 0.0 0.027393976749362232 0.0 10 0.0 0.0 0.0 0.027393976749362232 0.0 11 0.0 0.0 0.0 0.027393976749362232 0.0 12 0.0 0.0 0.0 0.027393976749362232 0.0 13 0.0 0.0 0.0 0.03253034738986765 0.0 14 0.0 0.0 0.0 0.03253034738986765 0.0 15 0.0 0.0 0.0 0.037666718030373074 0.0 16 0.0 0.0 0.0 0.037666718030373074 0.0 17 0.0 0.0 0.0 0.04622733576454877 0.0 18 0.0 0.0 0.0 0.06163644768606503 0.0 19 0.0 0.0 0.0 0.07875768315441642 0.0 20 0.0 0.0 0.0 0.07875768315441642 0.0 21 0.0 0.0 0.0 0.08731830088859212 0.0 22 0.0 0.0 0.0 0.09587891862276782 0.0 23 0.0 0.0 0.0 0.09587891862276782 0.0 24 0.0 0.0 0.0 0.09587891862276782 0.0 25 0.0 0.0 0.0 0.12669714246580033 0.0 26 0.0 0.0 0.0 0.14381837793415173 0.0 27 0.0 0.0 0.0 0.14553050148098687 0.0 28 0.0 0.0 0.0 0.147242625027822 0.0 29 0.0 0.0 0.0 0.17463660177718424 0.0 30 0.0 0.0 0.0 0.22257606108856814 0.0 31 0.0 0.0 0.0 0.2602427791189412 0.0 32 0.0 0.0 0.0 0.31331860907083053 0.0 33 0.0 0.0 0.0 0.34413683291386304 0.0 34 0.0 0.0 0.0 0.4006369099594227 0.0 35 0.0 0.0 0.0 0.5136370640505419 0.0 36 0.0 0.0 0.0 0.6882736658277261 0.0 37 0.0 0.0 0.0 0.9519406920403376 0.0 38 0.0 0.0 0.0 1.2327289537213004 0.0 39 0.0 0.0 0.0 1.5152293389490985 0.0 40 0.0 0.0 0.0 1.8165630831920832 0.0 41 0.0 0.0 0.0 2.2154878696046705 0.0 42 0.0 0.0 0.0 2.549351961237523 0.0 43 0.0 0.0 0.0 2.9482767476501106 0.0 44 0.0 0.0 0.0 3.2461862447994245 0.0 45 0.0 0.0 0.0 3.590323077713288 0.0 46 0.0 0.0 0.0 3.939596281267656 0.0 47 0.0 0.0 0.0 4.175869330730905 0.0 48 0.0 0.0 0.0 4.489187939801736 0.0 49 0.0 0.0 0.0 4.7734004485763695 0.0 50 0.0 0.0 0.0 5.143219134692759 0.0 51 0.0 0.0 0.0 5.334976971938295 0.0 52 0.0 0.0 0.0 5.538719674011677 0.0 53 0.0 0.0 0.0 5.7321896348040475 0.0 54 0.0 0.0 0.0 5.905114113034396 0.0 55 0.0 0.0 0.0 6.079750714811581 0.0 56 0.0 0.0 0.0 6.3074631465406545 0.0 57 0.0 0.0 0.0 6.50264523087986 0.0 58 0.0 0.0 0.0 6.667009091376034 0.0 59 0.0 0.0 0.0 6.773160751279812 0.0 60 0.0 0.0 0.0 6.937524611775986 0.0 61 0.0 0.0 0.0 7.069358124882291 0.0 62 0.0 0.0 0.0 7.18235827897341 0.0 63 0.0 0.0 0.0 7.324464533360727 0.0 64 0.0 0.0 0.0 7.440888934545517 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCATA 25 2.7961178E-6 70.047134 48 CTTCAGA 25 2.7961178E-6 70.047134 51 CGTCAAC 20 7.839638E-5 70.047134 48 CTGATTG 40 1.3096724E-10 70.047134 54 ATCTGAT 25 2.7961178E-6 70.047134 52 CAAAAAC 40 1.3096724E-10 70.047134 1 GTCATAA 15 0.0022084042 70.04713 49 GTATTAT 15 0.0022084042 70.04713 49 TCAACTG 15 0.0022084042 70.04713 50 ACATCAG 15 0.0022084042 70.04713 50 ATTATGC 15 0.0022084042 70.04713 51 GACATCA 15 0.0022084042 70.04713 49 AAAAGCG 15 0.0022084042 70.04713 67 CAGATTG 15 0.0022084042 70.04713 54 AATGTGG 15 0.0022084042 70.04713 69 AGTCATC 15 0.0022084042 70.04713 48 AAATGTG 15 0.0022084042 70.04713 68 AGAAGCA 20 7.873021E-5 69.98716 31 CAGTATT 20 7.873021E-5 69.98716 47 ACGCAGT 20 7.873021E-5 69.98716 27 >>END_MODULE