FastQCFastQC Report
Wed 25 May 2016
SRR1780919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242545
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3333913.745490527530974No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG2510.10348595106062793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG200.006949504452.4922685
TGCGGGT305.874443E-446.6597945
GTACATG404.5824403E-543.752581
CAGTTCC350.00125344740.0023571
CTCCGGT350.00125471939.994117
TCCGGTC350.00125471939.994118
GATGTCC556.684131E-638.1761938
GCCGTAT654.8310903E-737.7100854
CTCGGTG904.476533E-935.00206444
GTAGAAC400.002414984935.00206416
GCGCTTA400.002414984935.00206439
GATCATA501.705922E-435.002061
TCGGGAT501.707992E-434.9948434
CGGGATG501.707992E-434.9948435
ATGGTCA400.002417429834.9948439
TCGGTGG957.603376E-933.1530145
TCTCGGT851.0585609E-732.94311543
TCGCCGT751.4799498E-632.6820752
AGGTCGT652.0993371E-532.30293328
CATCGAG552.9891255E-431.81349623
Produced by FastQC (version 0.11.5)