FastQCFastQC Report
Wed 25 May 2016
SRR1780919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242545
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT213498.802077964913728No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG29011.1960667092704447No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC19670.8109835288297017No Hit
CATATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT11740.48403389061823576No Hit
ATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTCTT5190.21398091075882827RNA PCR Primer, Index 38 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4930.20326125048959987No Hit
CTTATACACATCTCCGCGCCCACGAGACATTTATCTATCTCGTATGCCGT4760.19625224185202744No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC3380.13935558349996907No Hit
CTAATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT3020.12451297697334514No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT2860.1179162629615123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTTT150.002223847469.9915412
CGAATGC550.063.62867441
AGGGGGG28900.063.2625370
CGTATGA601.8189894E-1258.32628641
ATATCGT553.6379788E-1157.2658137
GCCGTAT252.3862557E-456.00478446
TCGAATG750.055.9932440
GCCGTCT35950.054.13535746
CGTCTTC35850.054.0910948
CCGTCTT35650.054.0018147
ATGCCGT36650.053.8543444
TATGCCG36400.053.83965343
CGTATGC36500.053.7880241
TCGTATG37100.053.76708640
TGCCGTC36700.053.60131545
TCTCGTA36800.053.5397338
CTCGTAT36850.053.46708339
GTATGCC36550.053.427242
CTTGAAA36450.053.38175257
GTCTTCT36750.053.33788749