FastQCFastQC Report
Wed 25 May 2016
SRR1780918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186751
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2394412.821350354214973No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG4840.25916862560307574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG150.00222309269.991163
CGTTAAT200.006939077752.5074325
ATCTCGC200.006946434352.4933711
TCCCAAT200.006946434352.4933740
GCCGTAT552.2664608E-950.91629854
TGGTCGC604.9076334E-946.6607749
TCGGTGG851.8189894E-1245.3005345
GTCGCCG659.93532E-943.08302351
CGTATCA659.93532E-943.08302356
CGGTGGT855.638867E-1141.182346
TCGCCGT602.5813642E-740.83911552
CGCCGTA602.5813642E-740.83911553
CTCGGTG901.03682396E-1038.89439444
GTATCAT852.5520421E-937.0640757
GGTACTA400.002409416735.0143361
CGCCTTT400.00241258735.00495522
TCTCGGT1003.2196112E-1035.00495543
CCGCCTT400.00241258735.00495521
GTAGATC904.472895E-934.99558338
TCGCACC400.002415760434.9955814