FastQCFastQC Report
Wed 25 May 2016
SRR1780918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186751
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT145757.804509748274441No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG21711.1625105086452014No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC12350.661308373181402No Hit
CATATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT12210.6538117600441229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8470.4535450948053825No Hit
ATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTCTT3900.2088342231099164TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCGT2710.14511301144304448No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC2340.12530053386594983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT301.0198892E-769.99705544
CCGGTCG150.002222349469.9970559
CGAATGC150.002224711769.9783141
AGGGGGG19900.063.1211470
GCCGTCA453.8198777E-1062.219646
CTCGTAA401.0610165E-861.2474239
AGCCGTC352.963243E-759.99747545
TCGTAAG553.6379788E-1157.25498240
CAGTCCG509.767973E-1055.98264728
TGACGTC452.6881025E-854.4421545
CGTATGA452.6937414E-854.42757441
ATCTCGT25000.053.89773637
GATCTCG25300.053.81196636
TCTCGTA25500.053.80165538
CGTCTTC25050.053.79015448
CGATCTC25250.053.7799135
GCGATCT25350.053.7058334
TATGCCG25150.053.7010543
TGCCGTC25250.053.64130445
CCGTCTT25000.053.61774447