FastQCFastQC Report
Wed 25 May 2016
SRR1780917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757541
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293393.8729256898306494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT1500.037.33657552
TGGTCGC1650.036.0661249
GACCGTA501.7083986E-435.0053535
GCCGTAT1450.033.79603654
TCGGTGG1700.032.94403545
ACGCTTG450.004305713331.1138145
CGGTGGT1950.030.51546946
TACGGCG703.493514E-529.9946819
CTCGGTG1800.029.17112444
CGTGCGA604.9821E-429.16727310
GTCGCCG1950.028.7204451
CGGCTTA755.5797747E-528.0042835
GCGGGTA500.007203466328.00427835
GGTTATC500.007208111728.000581
ATCGCGT500.00721508527.9950356
GGTATAC1606.548362E-1126.2505441
CGGACTA1606.548362E-1126.24361224
CCGTGCG700.0012260225.0005199
ACGGCGG851.3100202E-424.70150220
CGCCGTA2001.8189894E-1224.50212553
Produced by FastQC (version 0.11.5)